Development and validation of a micromethod for fast quantification of 5-n-alkylresorcinols in grains and whole grain products

•A micromethod was developed for quantification of 5-n-alkylresorcinols (5nARs).•The 5nARs react with a Fast Blue RR salt (FBRR) in an alkaline alcoholic medium.•Readings of new method are highly correlated (R2=0.99) with a Fast Blue B method.•Bland–Altman plot suggests that both methods can be inte...

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Bibliographic Details
Published in:Food chemistry 2013-12, Vol.141 (4), p.3546-3551
Main Authors: Sampietro, D.A., Jimenez, C.M., Belizán, M.M., Vattuone, M.A., Catalán, C.A.N.
Format: Article
Language:English
Subjects:
Alkylresorcinols
Biological and medical sciences
Bran
Colorimetry - instrumentation
Colorimetry - methods
Edible Grain - chemistry
Fast Blue B
Fast Blue RR
Food toxicology
Medical sciences
Plant Extracts - chemistry
Resorcinols - chemistry
Toxicology
Whole-grain products
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Summary:•A micromethod was developed for quantification of 5-n-alkylresorcinols (5nARs).•The 5nARs react with a Fast Blue RR salt (FBRR) in an alkaline alcoholic medium.•Readings of new method are highly correlated (R2=0.99) with a Fast Blue B method.•Bland–Altman plot suggests that both methods can be interchangeably used.•The new micromethod saves time, reagents and solvents for quantification of 5nARs. A 96-well plate micromethod was developed to measure 5-n-alkylresorcinols (5nARs) in cereal grains and food derived products. The 5nARs reacted in alkaline alcoholic medium with Fast Blue RR ½ZnCl2 salt to yield coloured azo-derivatives. The highest sensitivity for 5nARs was obtained at 490nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. This reaction showed good linearity for olivetol (0.05–0.20μg). Contents of 5nARs determined in cereal grains and derived products by the new Fast Blue RR micromethod were highly correlated (R2=0.9944) with those obtained by a Fast Blue B method currently used. A Bland–Altman analysis indicated a small positive bias near to zero (R2=0.0401), suggesting that the methods can be interchangeably used. The new reaction is completed in 15min and the coloured products are read within the 15min after completion. The micromethod offers a fast analysis of 5nARs in cereal grains and derived products with low consumption of reagents and solvents.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2013.06.069