In vitro antifungal activity of topical and systemic antifungal drugs against Malassezia species

Summary The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility pro...

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Bibliographic Details
Published in:Mycoses 2013-09, Vol.56 (5), p.571-575
Main Authors: Carrillo-Muñoz, Alfonso Javier, Rojas, Florencia, Tur-Tur, Cristina, de los Ángeles Sosa, María, Diez, Gustavo Ortiz, Espada, Carmen Martín, Payá, María Jesús, Giusiano, Gustavo
Format: Article
Language:eng ; ger
Subjects:
Antifungal activity
Antifungal Agents - pharmacology
Dermatomycoses - microbiology
Humans
Malassezia
Malassezia - drug effects
Malassezia - genetics
Malassezia - isolation & purification
Malassezia furfur
Microbial Sensitivity Tests - methods
Molecular Typing
Mycological Typing Techniques
Polymorphism, Restriction Fragment Length
susceptibility
systemic antifungals
topical antifungals
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Summary:Summary The strict nutritional requirements of Malassezia species make it difficult to test the antifungal susceptibility. Treatments of the chronic and recurrent infections associated with Malassezia spp. are usually ineffective. The objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of Malassezia species against 16 antifungal drugs used for topical or systemic treatment. Isolates were identified by restriction fragment length polymorphism. Minimal inhibitory concentrations (MIC) were obtained by a modified microdilution method based on the Clinical Laboratory Standards Institute reference document M27‐A3. The modifications allowed a good growth of all tested species. High in vitro antifungal activity of most tested drugs was observed, especially triazole derivatives, except for fluconazole which presented the highest MICs and widest range of concentrations. Ketoconazole and itraconazole demonstrated a great activity. Higher MICs values were obtained with Malassezia furfur indicating a low susceptibility to most of the antifungal agents tested. Malassezia sympodialis and Malassezia pachydermatis were found to be more‐susceptible species than M. furfur, Malassezia globosa, Malassezia slooffiae and Malassezia restricta. Topical substances were also active but provide higher MICs than the compounds for systemic use. The differences observed in the antifungals activity and interspecies variability demonstrated the importance to studying the susceptibility profile of each species to obtain reliable information for defining an effective treatment regimen.
ISSN:0933-7407
1439-0507
DOI:10.1111/myc.12076