Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease

Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the current study was to analyze effec...

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Published in:American journal of physiology. Renal physiology 2013-08, Vol.305 (3), p.F314-F322
Main Authors: Patschan, Daniel, Schwarze, Katrin, Lange, Andrea, Meise, Nyree, Henze, Elvira, Becker, Jan Ulrich, Patschan, Susann, Müller, Gerhard A
Format: Article
Language:English
Subjects:
Acute Kidney Injury - pathology
Animals
Apoptosis - physiology
Bone Morphogenetic Protein 5 - physiology
Bones
Cell growth
Cell Movement - physiology
Cell Survival - physiology
Cells
Cells, Cultured
Endothelial Cells - physiology
Enzyme-Linked Immunosorbent Assay
Epithelial-Mesenchymal Transition - physiology
Inflammation Mediators - physiology
Kidney diseases
Kidney Function Tests
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Necrosis
Nephrectomy
Proteins
Proteinuria - metabolism
Renal Insufficiency, Chronic - pathology
Rodents
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Summary:Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the current study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5/6-nephrectomy. Male C57/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h [corrected] after cell therapy. In 5/6-nephrectomy, the cells failed to act renoprotectively, [corrected] but proteinuria was reduced after administering untreated eEOCs." Next, the original version read as "BMP-5 acts as a potent eEOC agonist in murine AKI in the short [corrected] term. Cell effects in 5/6-nephrectomy are heterogenous, but untreated cells act antifibrotically [corrected] without any impact on EnMT.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00677.2012