Isoflurane causes neocortical but not hippocampal-dependent memory impairment in mice

Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus‐dependent and neocortex‐dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the...

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Published in:Acta anaesthesiologica Scandinavica 2012-09, Vol.56 (8), p.1052-1057
Main Authors: FIDALGO, A. R., CIBELLI, M., WHITE, J. P. M., NAGY, I., WAN, Y., MA, D.
Format: Article
Language:English
Subjects:
Amnesia - chemically induced
Amnesia - psychology
Anesthesia
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Anesthetics, Inhalation - toxicity
Animals
Behavior, Animal - drug effects
Biological and medical sciences
Buprenorphine
Conditioning, Operant - drug effects
Electroshock
Fear - physiology
Hippocampus - physiopathology
Inflammation - physiopathology
Inflammation - psychology
Isoflurane - toxicity
Lipopolysaccharides
Male
Medical sciences
Memory Disorders - chemically induced
Memory Disorders - physiopathology
Mice
Mice, Inbred C57BL
Narcotics
Neocortex - physiopathology
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Summary:Background The aim of this study is to investigate the effect of general anaesthesia induced by isoflurane with buprenorphine on hippocampus‐dependent and neocortex‐dependent memory, respectively, in mice, and in addition, to compare the effects of such anaesthesia on these memory processes with the effects induced by lipopolysaccharide (LPS) administration on the same memory processes. Methods To assess hippocampus‐dependent memory, isoflurane (for 15 min) after buprenorphine injection, or LPS 100 μg/kg (intraperitoneally) was administered 24 h before or after fear conditioning. The effect of these treatments on hippocampus‐dependent memory was assessed using contextual fear‐conditioning tasks at day 4. To assess neocortex‐dependent memory, isoflurane anaesthesia or LPS was given 72 h after contextual fear conditioning. Neocortex‐dependent memory assessment was performed at day 32. Results Unlike LPS injection, isoflurane with buprenorphine‐induced anaesthesia does not impair freezing responses in hippocampus‐dependent fear‐conditioning memory tasks. On anterograde amnesia assessment: 49.67 ± 6.87% for the anaesthesia group and 54.5 ± 4.12% for the control group. On retrograde amnesia assessment: 47.16 ± 8.71% for the anaesthesia group and 54.5 ± 4.12% for control group; P > 0.05. Thus, neither isoflurane nor buprenorphine impair hippocampus‐dependent memory. However, on the neocortex‐dependent memory task, both isoflurane‐induced anaesthesia and LPS‐induced inflammation result in reduced freezing responses: 62.13 ± 5.80% for the anaesthesia group, 74.63 ± 5.69% for the LPS group, and 81.75 ± 3.26% for the control group; P 
ISSN:0001-5172
1399-6576
DOI:10.1111/j.1399-6576.2012.02691.x