Anti-inflammatory Effects of Oleanolic Acid on LPS-Induced Inflammation In Vitro and In Vivo

Oleanolic acid (OA) is a triterpenoid known for its anti-inflammatory and anti-cancer properties; however, the anti-inflammatory effects of OA on lipopolysaccharide (LPS)-mediated pro-inflammatory responses have not been studied. Here, we first investigated the possible anti-inflammatory effects of...

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Bibliographic Details
Published in:Inflammation 2013-02, Vol.36 (1), p.94-102
Main Authors: Lee, Wonhwa, Yang, Eun-Ju, Ku, Sae-Kwang, Song, Kyung-Sik, Bae, Jong-Sup
Format: Article
Language:English
Subjects:
Acetic Acid
Animals
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Biomedical and Life Sciences
Biomedicine
Carboxymethylcellulose Sodium
Cell Adhesion - drug effects
Cell Line
Cell Movement - drug effects
Cell Survival
E-Selectin
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Enzyme Activation - drug effects
Female
Human Umbilical Vein Endothelial Cells
Humans
Immunology
Inflammation - chemically induced
Inflammation - drug therapy
Intercellular Adhesion Molecule-1 - metabolism
Internal Medicine
Lipopolysaccharides
Mice
Mice, Inbred ICR
NF-kappa B - metabolism
Oleanolic Acid - pharmacology
Pathology
Pharmacology/Toxicology
Rheumatology
Transendothelial and Transepithelial Migration - drug effects
Tumor Necrosis Factor-alpha - biosynthesis
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:Oleanolic acid (OA) is a triterpenoid known for its anti-inflammatory and anti-cancer properties; however, the anti-inflammatory effects of OA on lipopolysaccharide (LPS)-mediated pro-inflammatory responses have not been studied. Here, we first investigated the possible anti-inflammatory effects of OA against pro-inflammatory responses in human umbilical vein endothelial cells (HUVECs) induced by LPS and the associated signaling pathways. We found that OA inhibited LPS-induced barrier disruption, expression of cell adhesion molecules (CAMs), and adhesion/transendothelial migration of monocytes to HUVECs. OA also suppressed acetic acid-induced hyperpermeability and carboxymethylcellulose-induced leukocyte migration in vivo . Further studies revealed that OA suppressed the production of tumor necrosis factor-α and activation of nuclear factor-κB by LPS. Collectively, these results suggest that OA has anti-inflammatory effects by inhibiting hyperpermeability, the expression of CAMs, and the adhesion and migration of leukocytes, thereby endorsing its usefulness as a therapeutic agent for vascular inflammatory diseases.
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-012-9523-9