Development of a transdermal Salmonella challenge model in calves

Recent investigations have found that Salmonella can be routinely recovered from peripheral lymph nodes (PLNs) of cattle presented for harvest. When contained within the PLNs, this foodborne pathogen is protected from currently used postharvest, inplant intervention strategies and, therefore, PLNs h...

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Bibliographic Details
Published in:Journal of food protection 2013-07, Vol.76 (7), p.1255-1258
Main Authors: Edrington, T. S, Loneragan, G. H, Hill, J, Genovese, K. J, He, H, Callaway, T. R, Anderson, R. C, Brichta-Harhay, D. M, Nisbet, D. J
Format: Article
Language:English
Subjects:
Animals
Beef
Biological and medical sciences
calves
Cattle
Cattle - microbiology
Colony Count, Microbial
Food contamination & poisoning
Food Contamination - analysis
Food Contamination - prevention & control
Food industries
Food Microbiology
food pathogens
Food safety
Fundamental and applied biological sciences. Psychology
Humans
Infections
Laboratories
lymph nodes
Lymph Nodes - microbiology
Lymphatic system
Meat Products - microbiology
Models, Animal
Pathogens
Prevalence
Salmonella
Salmonella - isolation & purification
salmonellosis
serotypes
Skin
transdermal application
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Summary:Recent investigations have found that Salmonella can be routinely recovered from peripheral lymph nodes (PLNs) of cattle presented for harvest. When contained within the PLNs, this foodborne pathogen is protected from currently used postharvest, inplant intervention strategies and, therefore, PLNs harboring Salmonella may be a potential contaminant of ground beef. The objective of this work was to develop a challenge model that effectively and repeatedly results in Salmonella-positive PLNs. A 10-lancet skin-allergy instrument was inoculated with Salmonella, and calves were inoculated intra- and/or transdermally by applying the device over various ventral regions of the skin. Salmonella was successfully and predictably recovered from regionspecific PLNs up to 8 days postchallenge. Furthermore, serotypes inoculated within specific regions were only recovered from the PLNs draining those regions. This model provides a method to predictably infect PLNs with Salmonella. Further, this model makes it possible to determine the duration of infection and to evaluate candidate interventions that may shorten the duration of infection.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X.JFP-12-317