Isolation, molecular detection and antibiogram of Listeria monocytogenes from human clinical cases and fish of Kashmir, India

The present study investigated the occurrence, antibiogram and molecular epidemiology of Listeria monocytogenes from fish and human clinical cases in Kashmir, India. A total of 100 fresh samples of commonly eaten indigenous fish, Cyprinus carpio and Schizothorax niger , were tested for the presence...

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Bibliographic Details
Published in:Comparative clinical pathology 2013-07, Vol.22 (4), p.661-665
Main Authors: Bhat, Shakoor Ahmad, Willayat, Malik Mehboob, Roy, Seuli Saha, Bhat, Mushtaq Ahmad, Shah, Shahid Nazir, Ahmed, Asif, Maqbool, Shahnawaz, Ganayi, Bashir Ahmd
Format: Article
Language:English
Subjects:
Cyprinus carpio
Freshwater
Hematology
Hyla
Listeria monocytogenes
Medicine
Medicine & Public Health
Oncology
Original Article
Pathology
Schizothorax niger
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Summary:The present study investigated the occurrence, antibiogram and molecular epidemiology of Listeria monocytogenes from fish and human clinical cases in Kashmir, India. A total of 100 fresh samples of commonly eaten indigenous fish, Cyprinus carpio and Schizothorax niger , were tested for the presence of L. monocytogenes. Clinical samples comprising of blood (40) and CSF (20) were taken from patients with signs of encephalitis, meningitis/meningoencephalitis, septicemia and pyrexia of unknown origin. Following enrichment and plating on selective agar, conformation of the organisms was done on the basis of various biochemical tests followed by Christie, Atkins, Munch-Peterson (CAMP) test. L. monocytogenes was isolated from two fish and two clinical samples (blood, 1; CSF, 1) showing an overall prevalence of 2.5%. The prevalence was 3.33% in clinical samples and 2.0% in fish samples. The isolates were subjected to PCR assay for virulence associated with hyl A genes. The pathogenicity of the isolates was confirmed by demonstration of monocytosis and kerato-conjunctivitis in rabbits. L. monocytogenes isolated from human clinical cases were sensitive to gentamicin, ciprofloxacin, doxycycline, ceftriaxone and resistant to ampicillin, amoxycillin, cefpodoxime, streptomycin, norfloxacin and cefphotaxime. However, the isolates recovered from foods were sensitive to gentamicin, doxycycline, enrofloxacin, amoxycillin/clavulanic acid, ciprofloxacin, ampicillin/cloxacillin, ceftriaxone and resistant to streptomycin, cefpodoxime and cefphotaxime. Multi-drug resistant strains were found in the present study, representing a potential threat to human health.
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-012-1462-1