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Knockdown of lipocalin-2 suppresses the growth and invasion of prostate cancer cells
BACKGROUND Lipocalin‐2 (LCN2) is a member of the lipocalin superfamily, and it has an important role in the regulation of cellular oncogenesis and apoptosis. However, the role for LCN2 in prostate cancer remains unclear. METHOD LCN2 expression has been determined by Western blotting, qRT‐PCR, and im...
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Published in: | The Prostate 2013-09, Vol.73 (12), p.1281-1290 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | BACKGROUND
Lipocalin‐2 (LCN2) is a member of the lipocalin superfamily, and it has an important role in the regulation of cellular oncogenesis and apoptosis. However, the role for LCN2 in prostate cancer remains unclear.
METHOD
LCN2 expression has been determined by Western blotting, qRT‐PCR, and immunohistochemistry in the human prostate cell lines PC3, DU145, LNCaP, and 22Rv, and in human prostate tissue array. In this study, we identified shRNA‐LCN2 to determine the role of LCN2 in prostate‐cancer cell proliferation, migration, and invasion. Cell proliferative ability was measured by MTT, colony‐formation, and cell‐cycle analysis. The role of LCN2 in prostate‐cancer cell migration and invasion was analyzed by cell‐migration assay and Matrigel invasion assay. The effect of LCN2 knockdown on prostate tumor growth was assessed in a subcutaneous xenograft model.
RESULTS
LCN2 protein and mRNA expression are higher in PC3 and DU145 cells than in LNCaP and 22Rv cells, and prostate cancer tissue correlated significantly with tumor differentiation (P |
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ISSN: | 0270-4137 1097-0045 |
DOI: | 10.1002/pros.22670 |