Knockdown of lipocalin-2 suppresses the growth and invasion of prostate cancer cells

BACKGROUND Lipocalin‐2 (LCN2) is a member of the lipocalin superfamily, and it has an important role in the regulation of cellular oncogenesis and apoptosis. However, the role for LCN2 in prostate cancer remains unclear. METHOD LCN2 expression has been determined by Western blotting, qRT‐PCR, and im...

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Bibliographic Details
Published in:The Prostate 2013-09, Vol.73 (12), p.1281-1290
Main Authors: Tung, Min-Che, Hsieh, Shu-Ching, Yang, Shun-Fa, Cheng, Chun-Wen, Tsai, Rong-Tzong, Wang, Shao-Chuan, Huang, Min-Hsien, Hsieh, Yi-Hsien
Format: Article
Language:English
Subjects:
Acute-Phase Proteins - deficiency
Acute-Phase Proteins - genetics
Aged
Animals
Cell Line, Tumor
Cell Proliferation
Down-Regulation - genetics
Gene Knockdown Techniques - methods
Humans
invasion
Lipocalin-2
Lipocalins - genetics
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
migration
Neoplasm Invasiveness - genetics
Neoplasm Invasiveness - pathology
proliferation
prostate
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Proto-Oncogene Proteins - deficiency
Proto-Oncogene Proteins - genetics
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Summary:BACKGROUND Lipocalin‐2 (LCN2) is a member of the lipocalin superfamily, and it has an important role in the regulation of cellular oncogenesis and apoptosis. However, the role for LCN2 in prostate cancer remains unclear. METHOD LCN2 expression has been determined by Western blotting, qRT‐PCR, and immunohistochemistry in the human prostate cell lines PC3, DU145, LNCaP, and 22Rv, and in human prostate tissue array. In this study, we identified shRNA‐LCN2 to determine the role of LCN2 in prostate‐cancer cell proliferation, migration, and invasion. Cell proliferative ability was measured by MTT, colony‐formation, and cell‐cycle analysis. The role of LCN2 in prostate‐cancer cell migration and invasion was analyzed by cell‐migration assay and Matrigel invasion assay. The effect of LCN2 knockdown on prostate tumor growth was assessed in a subcutaneous xenograft model. RESULTS LCN2 protein and mRNA expression are higher in PC3 and DU145 cells than in LNCaP and 22Rv cells, and prostate cancer tissue correlated significantly with tumor differentiation (P 
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.22670