The follicular thyroid cell line PCCL3 responds differently to laminin and to polylaminin, a polymer of laminin assembled in acidic pH

The extracellular-matrix protein laminin forms polymers both in vivo and in vitro. Acidification of pH leads to the formation of an artificial polymer with biomimetic properties, named polylaminin (polyLM). Follicle cells in the thyroid are in close contact with laminin, but their response to this i...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 2013-08, Vol.376 (1-2), p.12-22
Main Authors: Palmero, C.Y., Miranda-Alves, L., Sant’Ana Barroso, M.M., Souza, E.C.L., Machado, D.E., Palumbo-Junior, A., Santos, C.A.N., Portilho, D.M., Mermelstein, C.S., Takiya, C.M., Carvalho, D.P., Hochman-Mendez, C., Coelho-Sampaio, T., Nasciutti, L.E.
Format: Article
Language:English
Subjects:
acidification
actin
Actin Cytoskeleton - drug effects
Actin Cytoskeleton - metabolism
Actin Cytoskeleton - ultrastructure
Animals
Biological Transport
biomimetics
Cell Line
cell polarity
Cell Polarity - drug effects
Cell Proliferation - drug effects
Cell spreading
Cell Survival - drug effects
cultured cells
extracellular matrix
Gene Expression
glass
Hydrogen-Ion Concentration
Iodide uptake
iodides
Laminin
Laminin - pharmacology
microfilaments
PCCL3 cells
Peptides - chemistry
Peptides - pharmacology
phenotype
plasma membrane
Polylaminin
Polymerization
polymers
Rats
Rats, Inbred F344
Sodium Iodide - metabolism
Symporters - genetics
Symporters - metabolism
Thyroid
Thyroid Gland - cytology
Thyroid Gland - drug effects
Thyroid Gland - metabolism
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Summary:The extracellular-matrix protein laminin forms polymers both in vivo and in vitro. Acidification of pH leads to the formation of an artificial polymer with biomimetic properties, named polylaminin (polyLM). Follicle cells in the thyroid are in close contact with laminin, but their response to this important extracellular signal is still poorly understood. PCCL3 thyroid follicular cells cultured on glass, on regular laminin (LM) or on laminin previously polymerized in acidic pH (polyLM) showed different cell morphologies and propensities to proliferate, as well as differences in the organization of their actin cytoskeleton. On polyLM, cells displayed a typical epithelial morphology and radially organized actin fibers; whereas on LM, they spread irregularly on the substrate, lost cell contacts, and developed thick actin fibers extending through the entire cytoplasm. Iodide uptake decreased similarly in response to both laminin substrates, in comparison to glass. On both the LM and polyLM substrates, the expression of the sodium iodide symporter (NIS) decreased slightly but not significantly. NIS showed dotted immunostaining at the plasma membrane in the cells cultured on glass; on polyLM, NIS was observed mainly in the perinuclear region, and more diffusely throughout the cytoplasm on the LM substrate. Additionally, polyLM specifically favored the maintenance of cell polarity in culture. These findings indicate that PCCL3 cells can discriminate between LM and polyLM and that they respond to the latter by better preserving the phenotype observed in the thyroid tissue.
ISSN:0303-7207
1872-8057
DOI:10.1016/j.mce.2013.05.020