Global characterization of neutral saccharides in crude and processed Radix Rehmanniae by hydrophilic interaction liquid chromatography tandem electrospray ionization time-of-flight mass spectrometry

•It firstly developed a HILIC-MS method for simultaneous measurement of nine neutral saccharides.•Influences of column type, additive type, temperature and pH on analyte retention were evaluated.•This study revealed the practical value of TOFMS in quantitative saccharide profiling. The combined use...

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Bibliographic Details
Published in:Food chemistry 2013-12, Vol.141 (3), p.2833-2840
Main Authors: Liu, Zhirui, Lou, Ziyang, Ding, Xuan, Li, Xiang, Qi, Yunpeng, Zhu, Zhenyu, Chai, Yifeng
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carbohydrates - chemistry
Carbohydrates - isolation & purification
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Food toxicology
Hydrophilic interaction liquid chromatography (HILIC)
Hydrophobic and Hydrophilic Interactions
LC-TOFMS
Medical sciences
Neutral saccharides
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Radix Rehmanniae
Rehmannia - chemistry
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
Toxicology
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Summary:•It firstly developed a HILIC-MS method for simultaneous measurement of nine neutral saccharides.•Influences of column type, additive type, temperature and pH on analyte retention were evaluated.•This study revealed the practical value of TOFMS in quantitative saccharide profiling. The combined use of the state-of-the-art hybrid mass spectrometer (MS) together with high efficient liquid chromatography may prove to be a useful tool for neutral saccharide analysis. In the present work, we used hydrophilic interaction liquid chromatography (HILIC) to separate selected saccharides (fructose, glucose, galactose, sucrose, melibiose, raffinose, manninotriose, stachyose and verbascose). Influences of the column type, additive type, temperature, pH and other separation factors on analyte retention were evaluated. The new method did not require reduction, derivatization or postcolumn addition of reagents, which are commonly used in conventional saccharide analysis. Our results demonstrate the potential of HILIC-MS for sensitive and robust determination of saccharides in crude and processed Radix Rehmanniae, and may promote new perspectives in the research of other medicinal herbs.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2013.04.114