A nanosized metal-organic framework of Fe-MIL-88NH₂ as a novel peroxidase mimic used for colorimetric detection of glucose

In this paper, a nanosized porous metal-organic framework, Fe-MIL-88NH₂, was facilely prepared with a uniform octahedral shape by the addition of acetic acid, and for the first time was demonstrated to possess intrinsic peroxidase-like activity. Kinetic analysis and electron spin resonance measureme...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2013-08, Vol.138 (16), p.4526-4531
Main Authors: Liu, Ya Li, Zhao, Xi Juan, Yang, Xiao Xi, Li, Yuan Fang
Format: Article
Language:English
Subjects:
Colorimetry - methods
Glucose - analysis
Humans
Iron - chemistry
Metal Nanoparticles - chemistry
Peroxidase - chemistry
X-Ray Diffraction - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper, a nanosized porous metal-organic framework, Fe-MIL-88NH₂, was facilely prepared with a uniform octahedral shape by the addition of acetic acid, and for the first time was demonstrated to possess intrinsic peroxidase-like activity. Kinetic analysis and electron spin resonance measurements indicated that the catalytic behavior was consistent with typical Michaelis-Menten kinetics and follows a ping-pong mechanism. As a novel peroxidase mimic material, Fe-MIL-88NH₂ shows the advantages of high catalytic efficiency, ultrahigh stability and high biocompatibility in aqueous medium compared with natural enzymes and other peroxidase nanomimetics. Here, Fe-MIL-88NH₂ was used to quickly catalyze oxidation of the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to produce a colored product, which provided a simple, sensitive and selective method for the colorimetric detection of glucose. Glucose could be linearly detected in the range from 2.0 × 10⁻⁶ to 3.0 × 10⁻⁴ M with a detection limit of 4.8 × 10⁻⁷ M, and the color variation for glucose response was also obvious by visual observation at concentrations as low as 2.0 × 10⁻⁶ M. More importantly, the colorimetric method could be successfully applied to the determination of glucose in diluted serum samples.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an00560g