Antimicrobial and Antibiofilm Activity of Designed and Synthesized Antimicrobial Peptide, KABT-AMP

Lysine-rich peptide, designated as KABT-AMP, was designed and synthesized to supersede the irrational use of chemical antibiotics as standard therapy. KABT-AMP is a 22-amino acid helical cationic peptide (+10) and amphipathic in nature. The antimicrobial kinetics of the peptide was ascertained in th...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2013-07, Vol.170 (5), p.1184-1193
Main Authors: Thankappan, Bency, Jeyarajan, Sivakumar, Hiroaki, Sakaue, Anbarasu, Kumarasamy, Natarajaseenivasan, Kalimuthusamy, Fujii, Noriko
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemical synthesis
Anti-Bacterial Agents - pharmacology
Antimicrobial Cationic Peptides - chemical synthesis
Antimicrobial Cationic Peptides - pharmacology
Bacterial Physiological Phenomena - drug effects
Biochemistry
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biological and medical sciences
Biotechnology
Candida albicans
Candida tropicalis
Chemical synthesis
Chemistry
Chemistry and Materials Science
Escherichia coli
Fundamental and applied biological sciences. Psychology
Peptides
Staphylococcus aureus
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Summary:Lysine-rich peptide, designated as KABT-AMP, was designed and synthesized to supersede the irrational use of chemical antibiotics as standard therapy. KABT-AMP is a 22-amino acid helical cationic peptide (+10) and amphipathic in nature. The antimicrobial kinetics of the peptide was ascertained in the representative strains of gram-positive, gram-negative, and fungal strains, viz., Staphylococcus aureus MTCC 2940, Escherichia coli MTCC 2939, and Candida albicans MTCC 227, respectively. KABT-AMP was synthesized by solid-phase synthesis and purified using reverse-phase high-performance liquid chromatography which resulted in >95 % purity, and matrix-assisted laser desorption/ionization time of flight revealed the mass of the peptide to be 2.8 kDa. KABT-AMP showed significant broad-spectrum antimicrobial activity against the bacterial and fungal strains analyzed in the present study with survivability of 30.8, 30.6, and 31.7 % in E. coli , S. aureus , and C. albicans , respectively, at 6 h. KABT-AMP also demonstrated antibiofilm activity against the tested biofilm forming clinical isolate, Candida tropicalis . The putative membranolytic activity of the peptide was substantiated by electron microscopic analysis. Results reveal that KABT-AMP will exhibit noteworthy antimicrobial activity against multidrug-resistant bacteria and fungus at micromolar concentrations with minimal cytotoxicity and thus could be conceived for biomedical application.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-013-0258-3