Online solid-phase extraction–liquid chromatography–electrospray–tandem mass spectrometry determination of multiple classes of antibiotics in environmental and treated waters

An online solid-phase extraction and liquid chromatography in combination with tandem mass spectrometry method was developed for the simultaneous determination of 31 antibiotics in drinking water, surface water and reclaimed waters. The developed methodology requires small sample volume (10 mL), ver...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2013-07, Vol.405 (18), p.5953-5964
Main Authors: Panditi, Venkata R., Batchu, Sudha Rani, Gardinali, Piero R.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anti-Bacterial Agents - analysis
Antibiotics
Biochemistry
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, Liquid - methods
Drinking water
Drinking Water - analysis
Erythromycin - analysis
Extraction (Chemistry)
Florida
Food Science
Fresh Water - analysis
High-Throughput Screening Assays - methods
Identification and classification
Laboratory Medicine
Limit of Detection
Liquid chromatography
Mass spectrometry
Methods
Monitoring/Environmental Analysis
Online Systems
Original Paper
Pharmaceutical chemistry
Rivers - chemistry
Scientific imaging
Solid Phase Extraction - methods
Spectrometry, Mass, Electrospray Ionization - methods
Surface water
Tandem Mass Spectrometry - methods
Water chemistry
Water Pollutants, Chemical - analysis
Water Purification - methods
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Summary:An online solid-phase extraction and liquid chromatography in combination with tandem mass spectrometry method was developed for the simultaneous determination of 31 antibiotics in drinking water, surface water and reclaimed waters. The developed methodology requires small sample volume (10 mL), very little sample preparation and total sample run time was 20 min. An Ion Max API heated electrospray ionization source operated in the positive mode with two selected reaction monitoring transitions was used per antibiotic for positive identity and quantification performed by the internal standard approach, to correct for matrix effects and any losses in the online extraction step. Method detection limits were in the range of 1.2–9.7, 2.2–15, 5.5–63 ng/L in drinking water, surface water and reclaimed waters, respectively. The method accuracy in matrix spiked samples ranged from 50–150 % for the studied antibiotics. The applicability of the method was demonstrated using various environmental and reclaimed water matrices. Erythromycin was detected in more than 85 % of the samples in all matrices (28–414, n.d.–199, n.d.–66 ng/L in reclaimed, river and drinking waters respectively). The other frequently detected antibiotics in reclaimed waters were nalidixic acid, clarithromycin, azithromycin, trimethoprim, and sulfamethoxazole.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-013-6863-8