Nitrogen-Fixing and Uricolytic Bacteria Associated with the Gut of Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scolytinae)

The bark beetles of the genus Dendroctonus feed on phloem that is a nitrogen-limited source. Nitrogen fixation and nitrogen recycling may compensate or alleviate such a limitation, and beetle-associated bacteria capable of such processes were identified. Raoultella terrigena, a diazotrophic bacteria...

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Bibliographic Details
Published in:Microbial ecology 2013-07, Vol.66 (1), p.200-210
Main Authors: Morales-Jiménez, Jesús, de León, Arturo Vera-Ponce, García-Domínguez, Aidé, Martínez-Romero, Esperanza, Zúñiga, Gerardo, Hernández-Rodríguez, César
Format: Article
Language:English
Subjects:
Adult insects
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Bacterial Proteins - genetics
Bark beetles
Biological and medical sciences
Biomedical and Life Sciences
Coleoptera - classification
Coleoptera - growth & development
Coleoptera - microbiology
Curculionidae
Dendroctonus
Dendroctonus valens
Ecology
Female
Female animals
Fundamental and applied biological sciences. Psychology
Gastrointestinal Tract - microbiology
Geoecology/Natural Processes
Insect larvae
INVERTEBRATE MICROBIOLOGY
Larva - classification
Larva - growth & development
Larva - microbiology
Life Sciences
Male
Male animals
Microbial Ecology
Microbiology
Molecular Sequence Data
Nature Conservation
Nitrogen
Nitrogen - metabolism
Nitrogen Fixation
Nitrogenase - genetics
Phloem
Phylogeny
Pseudomonas fluorescens
Rahnella aquatilis
Raoultella terrigena
Rhizophagus
Scolytidae
Scolytinae
Serratia
Uric acid
Water Quality/Water Pollution
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Summary:The bark beetles of the genus Dendroctonus feed on phloem that is a nitrogen-limited source. Nitrogen fixation and nitrogen recycling may compensate or alleviate such a limitation, and beetle-associated bacteria capable of such processes were identified. Raoultella terrigena, a diazotrophic bacteria present in the gut of Dendroctonus rhizophagus and D. valens, exhibited high acetylene reduction activity in vitro with different carbon sources, and its nifH and nifD genes were sequenced. Bacteria able to recycle uric acid were Pseudomonas fluorescens DVL3A that used it as carbon and nitrogen source, Serratia proteomaculans 2A CDF and Rahnella aquatilis 6-DR that used uric acid as sole nitrogen source. Also, this is the first report about the uric acid content in whole eggs, larvae, and adults (male and female) samples of the red turpentine beetle (Dendroctonus valens). Our results suggest that the gut bacteria of these bark beetles could contribute to insect N balance.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-013-0206-3