An aptamer based surface plasmon resonance biosensor for the detection of bovine catalase in milk

In this research, we report the development of an aptamer based SPR biosensor for the detection of catalase in milk samples with minimal sample preparation. A biotin tagged aptamer was immobilized onto a gold surface by affinity capture. A limit of detection (LOD) in the nanomolar range (20.5nM, RSD...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2013-10, Vol.48, p.126-131
Main Authors: Ashley, Jon, Li, Sam F.Y.
Format: Article
Language:English
Subjects:
Animals
Aptamer
Aptamers, Nucleotide - chemistry
Biological and medical sciences
Biosensors
Biotechnology
Bovine catalase
Catalase - analysis
Cattle
Enzyme Assays - methods
Fundamental and applied biological sciences. Psychology
Limit of Detection
Methods. Procedures. Technologies
Milk
Milk - enzymology
SPR
Surface plasmon resonance
Surface Plasmon Resonance - methods
Various methods and equipments
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Summary:In this research, we report the development of an aptamer based SPR biosensor for the detection of catalase in milk samples with minimal sample preparation. A biotin tagged aptamer was immobilized onto a gold surface by affinity capture. A limit of detection (LOD) in the nanomolar range (20.5nM, RSD: 15.2%) was found and a dynamic range of 15–1000nM was established for catalase in buffer and the aptamer showed good specificity toward catalase. This biosensor has the potential to be used in the detection of catalase in milk samples, a key indicator of mastitis disease in milk. •We developed a catalse aptamer-based surface plasmon resonance (SPR) biosensor.•The sensor was used for detection of catalase in milk samples.•A limit of detection (LOD) in the nanomolar range (20.5nM) was found.•A dynamic range of 15–1000nM was established.•This biosensor could be used for detection of catalase, a key indicator of mastitis disease.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2013.04.009