New Insights into the Mechanisms of Ram Sperm Protection by Seminal Plasma Proteins

To provide new insights into the mechanisms through which seminal plasma proteins (SPP) are able to protect spermatozoa, we tested the hypothesis that apoptosis can contribute to the negative effect of refrigeration on ram spermatozoa, and that SPP prevent this damage. Having proved the presence of...

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Bibliographic Details
Published in:Biology of reproduction 2013-06, Vol.88 (6), p.149-149
Main Authors: MENDOZA, Noelia, CASAO, Adriana, PEREZ-PE, Rosaura, CEBRIAN-PEREZ, José A, MUINO-BLANCO, Teresa
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Apoptosis - physiology
Biological and medical sciences
Cell Survival - drug effects
Cell Survival - physiology
Fundamental and applied biological sciences. Psychology
Male
Refrigeration
Semen Preservation - methods
Seminal Plasma Proteins - pharmacology
Sheep
Sperm Capacitation - drug effects
Sperm Capacitation - physiology
Sperm Motility - drug effects
Sperm Motility - physiology
Spermatozoa - drug effects
Spermatozoa - metabolism
Vertebrates: reproduction
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Summary:To provide new insights into the mechanisms through which seminal plasma proteins (SPP) are able to protect spermatozoa, we tested the hypothesis that apoptosis can contribute to the negative effect of refrigeration on ram spermatozoa, and that SPP prevent this damage. Having proved the presence of key constituents of apoptosis-related pathways in ram sperm protein extracts, we carried out a comparative analysis of the effects of the addition of SPP before refrigeration (15 °C, 30 min) and induced-apoptosis with betulinic acid or fibroblast-associated receptor ligand, assessing sperm quality parameters and apoptotic markers. The protective effect of SPP on plasma membrane integrity and potential, motility and mitochondrial inner membrane potential, and surface (cardiolipin content) was evidenced in refrigerated and induced-apoptosis samples. The addition of SPP resulted in lower values of phosphatidylserine externalization, DNA damage, and caspase activity. Therefore, apoptosis in fresh or refrigerated ram spermatozoa can occur due to activation of both the extrinsic and the intrinsic mediated pathway, and SPP might interfere with both pathways. The addition of SPP also resulted in higher proportions of viable, noncapacitated sperm and fertilizing ability (ZBA rate). This report demonstrates that SPP support survival of ram spermatozoa acting not only at the plasma membrane but also by inhibition of capacitation, and proposes the possibility that SPP might interfere with the extrinsic and the intrinsic apoptotic pathways. This opens new, interesting perspectives for the study of cellular regulatory mechanisms in spermatozoa that could be crucial for the improvement of ram semen preservation protocols.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod.112.105650