The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development

In order to understand the molecular genetic mechanisms of rice (Oryza sativa) organ development, we studied the narrow leaf2 narrow leaf3 (nal2 nal3; hereafter nal2/3) double mutant, which produces narrow-curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow-thin grains. We f...

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Bibliographic Details
Published in:The New phytologist 2013-06, Vol.198 (4), p.1071-1084
Main Authors: Cho, Sung‐Hwan, Yoo, Soo‐Cheul, Zhang, Haitao, Pandeya, Devendra, Koh, Hee‐Jong, Hwang, Ji‐Young, Kim, Gyung‐Tae, Paek, Nam‐Chon
Format: Article
Language:English
Subjects:
Arabidopsis
auxin
Auxins
Base Sequence
Body organs
Body Patterning - genetics
Cloning
Cloning, Molecular
Corn
Developmental biology
Gene Expression Profiling
Gene expression regulation
Gene Expression Regulation, Plant
Genes
Genes, Plant - genetics
Genetic Loci - genetics
Grain
Homeobox
Homology
lateral root
Leaf blade
Leaves
Molecular Sequence Data
Morphogenesis
Mutants
Mutation - genetics
nal2 nal3
narrow‐curly leaf
narrow‐thin spikelet
Nuclear Proteins - metabolism
Organs
Oryza - anatomy & histology
Oryza - growth & development
Oryza - metabolism
Oryza - ultrastructure
Oryza sativa
OsWOX3A
Palea
Phenotype
Phenotypes
Plant Leaves - growth & development
Plant Leaves - metabolism
Plant Leaves - ultrastructure
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - growth & development
Plant tissues
Plant Vascular Bundle - growth & development
Plants
Rice
rice (Oryza sativa)
RNA, Messenger - genetics
RNA, Messenger - metabolism
Root development
Roots
Spikelets
tiller
Tillers
Trans-Activators - metabolism
Transcription
Vascular tissue
Zea mays
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Summary:In order to understand the molecular genetic mechanisms of rice (Oryza sativa) organ development, we studied the narrow leaf2 narrow leaf3 (nal2 nal3; hereafter nal2/3) double mutant, which produces narrow-curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow-thin grains. We found that narrow-curly leaves resulted mainly from reduced lateral-axis outgrowth with fewer longitudinal veins and more, larger bulliform cells. Opened spikelets, possibly caused by marginal deformity in the lemma, gave rise to narrow-thin grains. Map-based cloning revealed that NAL2 and NAL3 are paralogs that encode an identical OsWOX3A (OsNS) transcriptional activator, homologous to NARROW SHEATH1 (NS1) and NS2 in maize and PRESSED FLOWER in Arabidopsis. OsWOX3A is expressed in the vascular tissues of various organs, where nal2/3 mutant phenotypes were displayed. Expression levels of several leaf development-associated genes were altered in nal2/3, and auxin transportrelated genes were significantly changed, leading to pin mutant-like phenotypes such as more tillers and fewer lateral roots. OsWOX3A is involved in organ development in rice, lateral-axis outgrowth and vascular patterning in leaves, lemma and palea morphogenesis in spikelets, and development of tillers and lateral roots.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.12231