ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae

•PDR1 R821S mutant had tolerance to a hydrophilic organic solvent, as well as hydrophobic.•We identified ABC transporters and cell wall proteins involved in organic solvent tolerance.•The proteins involved in organic solvent tolerance varied according to solvent properties.•The improvement of tolera...

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Bibliographic Details
Published in:Journal of biotechnology 2013-05, Vol.165 (2), p.145-152
Main Authors: Nishida, Nao, Ozato, Naoki, Matsui, Ken, Kuroda, Kouichi, Ueda, Mitsuyoshi
Format: Article
Language:English
Subjects:
ABC transporter
ABC transporters
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
biotechnology
Cell Wall - drug effects
Cell Wall - metabolism
Cell wall protein
dimethyl sulfoxide
diploidy
gene expression regulation
gene overexpression
genes
haploidy
hydrophilicity
hydrophobicity
multiple drug resistance
mutants
Organic solvent tolerance
point mutation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
solvents
Solvents - pharmacology
structural proteins
transcription factors
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Summary:•PDR1 R821S mutant had tolerance to a hydrophilic organic solvent, as well as hydrophobic.•We identified ABC transporters and cell wall proteins involved in organic solvent tolerance.•The proteins involved in organic solvent tolerance varied according to solvent properties.•The improvement of tolerance to specific organic solvents would contribute to the practical use. Pleiotropic drug resistance 1 (Pdr1p) protein is a key transcription factor in multidrug resistance. Specifically, R821S point mutation in the PDR1 gene in Saccharomyces cerevisiae diploid KK-211 strain plays an important role in tolerance to hydrophobic organic solvents, though the molecular mechanisms underlying organic solvent tolerance are unclear. In KK-211, several ABC transporters and cell wall proteins were upregulated. PDR1 R821S mutant derived from the laboratory haploid strain MT8-1 confirmatively tolerated hydrophobic organic solvents, and this study is the first to reveal its tolerance of the hydrophilic organic solvent, dimethyl sulfoxide (DMSO). To identify the genes involved in the organic solvent tolerance, we focused on the upregulated 4 ABC transporters and 6 cell wall proteins in KK-211, and demonstrated 2 ABC transporters, Pdr10p and Snq2p, and the 4 cell wall proteins, Wsc3p, Pry3p, Pir1p, and Ynl190wp were responsible for hydrophobic organic solvent (n-decane and n-undecane) tolerance. Tolerance to the hydrophilic organic solvent, DMSO, was facilitated by the overproduction of 2 ABC transporters, Snq2p and Yor1p and, 3 cell wall proteins, Wsc3p, Pry3p, and Ynl190wp. Our results suggest that overexpression of genes encoding proteins effective for tolerance to specific organic solvents would enable enhanced tolerances for practical use.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2013.03.003