Genetic diversity of the mitochondrial cytochrome b gene in Lutzomyia spp., with special reference to Lutzomyia peruensis, a main vector of Leishmania (Viannia) peruviana in the Peruvian Andes

Haplotype network of the cytochrome b sequences of Lutzomyia peruensis collected from 9 Andean areas in Peru. [Display omitted] ► Cytochrome b (cyt b) gene sequences were determined for 13 sand fly species in Peru. ► Highly polymorphic sites were identified in the middle region of the cyt b gene. ►...

Full description

Saved in:
Bibliographic Details
Published in:Acta tropica 2013-05, Vol.126 (2), p.156-163
Main Authors: Yamamoto, Kento, Cáceres, Abraham G., Gomez, Eduardo A., Mimori, Tatsuyuki, Iwata, Hiroyuki, Korenaga, Masataka, Sakurai, Tatsuya, Katakura, Ken, Hashiguchi, Yoshihisa, Kato, Hirotomo
Format: Article
Language:English
Subjects:
Animals
Arthropoda
arthropods
Base Sequence
Cytochrome b
Cytochromes b - genetics
genes
genetic drift
genetic techniques and protocols
Genetic Variation
Genetics, Population
Genotype
Geography
Haplotype
Haplotypes
Insect Vectors - enzymology
Insect Vectors - genetics
Insect Vectors - parasitology
insecticides
Leishmania
Leishmania - physiology
Leishmaniasis - epidemiology
Leishmaniasis - transmission
Lutzomyia
Mitochondrial Proteins - genetics
Molecular Sequence Data
nucleotide sequences
Peru - epidemiology
Phylogeny
Psychodidae - enzymology
Psychodidae - genetics
Psychodidae - parasitology
Sand fly
Sequence Analysis, DNA
vectorial capacity
Viannia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Haplotype network of the cytochrome b sequences of Lutzomyia peruensis collected from 9 Andean areas in Peru. [Display omitted] ► Cytochrome b (cyt b) gene sequences were determined for 13 sand fly species in Peru. ► Highly polymorphic sites were identified in the middle region of the cyt b gene. ► Haplotype analysis showed area-dependent genetic polymorphism in Lutzomyia peruensis. ► The cyt b gene will be a useful marker for genetic structure in sand fly populations. The genetic divergence caused by genetic drift and/or selection is suggested to affect the vectorial capacity and insecticide susceptibility of sand flies, as well as other arthropods. In the present study, cytochrome b (cyt b) gene sequences were determined in 13 species circulating in Peru to establish a basis for analysis of the genetic structure, and the intraspecific genetic diversity was assessed in the Lutzomyia (Lu.) peruensis, a main vector species of Leishmania (Viannia) peruviana in Peruvian Andes. Analysis of intraspecific genetic diversity in the cyt b gene sequences from 36 Lu. peruensis identified 3 highly polymorphic sites in the middle region of the gene. Haplotype and gene network analyses were performed on the cyt b gene sequences of 130 Lu. peruensis in 9 Andean areas from 3 Departments (Ancash, Lima and La Libertad). The results showed that the populations of La Libertad were highly polymorphic and that their haplotypes were distinct from those of Ancash and Lima, where dominant haplotypes were observed, suggesting that a population bottleneck may have occurred in Ancash and Lima, but not in La Libertad. The present study indicated that the middle region of the cyt b gene is useful for the analysis of genetic structure in sand fly populations.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2013.02.007