Rates of dinitrogen fixation and the abundance of diazotrophs in North American coastal waters between Cape Hatteras and Georges Bank

We coupled dinitrogen (N₂) fixation rate estimates with molecular biological methods to determine the activity and abundance of diazotrophs in coastal waters along the temperate North American Mid-Atlantic continental shelf during multiple seasons and cruises. Volumetric rates of N₂ fixation were as...

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Bibliographic Details
Published in:Limnology and oceanography 2012-07, Vol.57 (4), p.1067-1083
Main Authors: Mulholland, M. R., Bernhardt, P. W., Blanco-Garcia, J. L., Mannino, A., Hyde, K., Mondragon, E., Turk, K., Moisander, P. H., Zehr, J. P.
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects
Marine
Synecology
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Summary:We coupled dinitrogen (N₂) fixation rate estimates with molecular biological methods to determine the activity and abundance of diazotrophs in coastal waters along the temperate North American Mid-Atlantic continental shelf during multiple seasons and cruises. Volumetric rates of N₂ fixation were as high as 49.8 nmol N L−1 d−1 and areal rates as high as 837.9 μmol N m−2 d−1 in our study area. Our results suggest that N₂ fixation occurs at high rates in coastal shelf waters that were previously thought to be unimportant sites of N₂ fixation and so were excluded from calculations of pelagic marine N₂ fixation. Unicellular N₂-fixing group A cyanobacteria were the most abundant diazotrophs in the Atlantic coastal waters and their abundance was comparable to, or higher than, that measured in oceanic regimes where they were discovered. High rates of N₂ fixation and the high abundance of diazotrophs along the North American Mid-Atlantic continental shelf highlight the need to revise marine N budgets to include coastal N₂ fixation. Integrating areal rates of N₂ fixation over the continental shelf area between Cape Hatteras and Nova Scotia, the estimated N₂ fixation in this temperate shelf system is about 0.02 Tmol N yr−1, the amount previously calculated for the entire North Atlantic continental shelf. Additional studies should provide spatially, temporally, and seasonally resolved rate estimates from coastal systems to better constrain N inputs via N₂ fixation from the neritic zone.
ISSN:0024-3590
1939-5590
DOI:10.4319/lo.2012.57.4.1067