Involvement of DNA methylation in the regulation of STS10 gene expression in Vitis amurensis

DNA methylation is known to play an important role in various developmental processes and defense mechanisms in plants and other organisms. However, it is not known whether DNA methylation is implicated in the genetic regulation of plant secondary metabolism, including resveratrol biosynthesis. Resv...

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Bibliographic Details
Published in:Planta 2013-04, Vol.237 (4), p.933-941
Main Authors: Kiselev, Konstantin V., Tyunin, Alexey P., Zhuravlev, Yuri N.
Format: Article
Language:English
Subjects:
Actins - metabolism
Acyltransferases - genetics
Acyltransferases - metabolism
Agriculture
Agrobacterium rhizogenes
Arachis hypogaea
Biomedical and Life Sciences
Callus
Cell culture techniques
Cultured cells
DNA
DNA Methylation
Ecology
Forestry
Gene expression
Gene Expression Regulation, Plant
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Life Sciences
Methylation
Open reading frames
Original Article
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Sciences
Plants
Promoter regions
Stilbenes
Stilbenes - metabolism
Vitaceae
Vitis
Vitis - genetics
Vitis - metabolism
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Summary:DNA methylation is known to play an important role in various developmental processes and defense mechanisms in plants and other organisms. However, it is not known whether DNA methylation is implicated in the genetic regulation of plant secondary metabolism, including resveratrol biosynthesis. Resveratrol is a naturally occurring polyphenol that is present in grapes, peanuts, and other plant sources, and it exhibits a wide range of valuable biologically active properties. The transformation of the wild-growing grape Vitis amurensis with the oncogene rolB from Agrobacterium rhizogenes has been demonstrated to considerably increase resveratrol production. To investigate whether DNA methylation regulates resveratrol biosynthesis, we treated both rolB transgenic and empty vector control V. amurensis cell cultures with the DNA demethylation agent 5-azacytosine (azaC). The azaC treatment significantly increased stilbene synthase 10 gene (VaSTS10) expression and resveratrol content in the V. amurensis cell cultures. Using bisulfite sequencing, we examined the methylation status of VaSTS10 in cell cultures under normal conditions and after azaC treatment. Both the promoter and 3'-end of the protein coding region of the VaSTS10 gene were hypermethylated (54-67 %) in the control cell culture. The rolB transgenic cell culture had high levels of resveratrol and lower hypermethylation levels of the VaSTS10 gene (20-47 %). The azaC treatment resulted in reduction in the DNA methylation levels in the promoter and coding regions of the VaSTS10 gene in both cell cultures. These data suggest that the DNA methylation may be involved in the control of resveratrol biosynthesis via the regulation of STS genes expression.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-012-1806-8