Novel mutations in the antifolate drug resistance marker genes among Plasmodium vivax isolates exhibiting severe manifestations

[Display omitted] ► Novel mutations were observed in both DHFR and DHPS of Plasmodium vivax. ► Presence of novel mutations in PvDHPS gene suggests its polymorphic nature. ► Frequency of PvDHFR–PvDHPS two locus mutations were higher among the severe isolates. Plasmodium vivax is the predominant speci...

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Bibliographic Details
Published in:Experimental parasitology 2012-12, Vol.132 (4), p.410-416
Main Authors: Garg, Shilpi, Saxena, Vishal, Lumb, Vanshika, Pakalapati, Deepak, Boopathi, P.A., Subudhi, Amit Kumar, Chowdhury, Shibasish, Kochar, Sanjay K., Kochar, Dhanpat K., Sharma, Y.D., Das, Ashis
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
alleles
Antifolate drug resistance
chloroquine
Chloroquine - pharmacology
Chloroquine - therapeutic use
Dihydropteroate Synthase - genetics
drug resistance
Drug Resistance - genetics
drugs
Female
Folic Acid Antagonists - pharmacology
Folic Acid Antagonists - therapeutic use
genetic markers
Genetic Markers - genetics
Genotype
Humans
India
loci
malaria
Malaria, Vivax - blood
Malaria, Vivax - drug therapy
Malaria, Vivax - parasitology
Male
Middle Aged
molecular models
mutants
Mutation
Novel mutations
parasites
parasitology
patients
Plasmodium falciparum
Plasmodium vivax
Plasmodium vivax - drug effects
Plasmodium vivax - genetics
Polymorphism, Genetic
Severe manifestations
Tetrahydrofolate Dehydrogenase - genetics
Young Adult
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Summary:[Display omitted] ► Novel mutations were observed in both DHFR and DHPS of Plasmodium vivax. ► Presence of novel mutations in PvDHPS gene suggests its polymorphic nature. ► Frequency of PvDHFR–PvDHPS two locus mutations were higher among the severe isolates. Plasmodium vivax is the predominant species of the human malaria parasite present in the Indian subcontinent. There have been recent reports on Chloroquine (CQ) resistance and severe manifestations shown by P. vivax from different regions of the world including India. This study focuses on Bikaner, India where during the last few years there have been continuous reports of severe manifestations by both Plasmodium falciparum and P. vivax. This region has a widespread use of Chloroquine and Sulfadoxine–Pyrimethamine for the treatment of malaria, but the resistance profiles of these drugs are not available. We report here the profile of mutations in marker genes associated with Chloroquine and antifolate drug resistance among the P. vivax parasites obtained from patients with severe (n=30) and non-severe (n=48) manifestations from this region. Most isolates showed the wild type alleles for both the Chloroquine and antifolate resistance markers (P
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2012.09.018