Isolation, characterisation and biological evaluation of a phenoxazine, a natural dyestuff isolated from leaves of Peristrophe bivalvis

Peristrophe bivalvis (L.) Merr. (Acanthaceae) is a wild growing and cultivated plant used for dyeing of foods by the ethnic minorities of Vietnam. The major component of the colour aqueous extract (CAE) of its leaves was identified as peristrophine by spectral analysis, especially the 2D NMR spectra...

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Bibliographic Details
Published in:Natural product research 2013-04, Vol.27 (8), p.771-774
Main Authors: Thuy, Trinh Thi, Huong, Nguyen Thi Thanh, Nhung, Le Thi Hong, Ninh, Pham Thi, Delfino, Domenico V., Van Sung, Tran
Format: Article
Language:English
Subjects:
Acanthaceae - chemistry
Acanthaceae - toxicity
Animals
Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Coloring Agents - chemistry
Coloring Agents - isolation & purification
Drug Screening Assays, Antitumor
Hep G2 Cells
Humans
Magnetic Resonance Spectroscopy
Mice
Molecular Structure
natural dyestuff phenoxazine
Oxazines - chemistry
Oxazines - isolation & purification
P. roxburghiana
Peristrophe bivalvis
peristrophine
Plant Leaves - chemistry
Spectrometry, Mass, Electrospray Ionization
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Summary:Peristrophe bivalvis (L.) Merr. (Acanthaceae) is a wild growing and cultivated plant used for dyeing of foods by the ethnic minorities of Vietnam. The major component of the colour aqueous extract (CAE) of its leaves was identified as peristrophine by spectral analysis, especially the 2D NMR spectra (HSQC, HMBC and NOESY). Considering the widespread utilisation of the decoction of this plant for food dyeing, we evaluated the acute oral toxicity of the CAE. Based on the results in an acute toxicity study in mice, the LD 50 value of the CAE was determined as 9100 ± 290 mg kg −1 body weight. Additionally, in vitro cytotoxic assay showed an inhibition of peristrophine against Hepatocellular carcinoma (HepG2, IC 50 3.90 µg mL −1 ). CAE and peristrophine (1) have also been tested for their ability to affect the cell number of the OCI acute myeloid leukaemia cell line. CAE and peristrophine significantly decreased the OCI cell number at different concentrations and times of treatment.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2012.698409