Growth kinetics and immune response of chimeric foot-and-mouth disease virus serotype ‘O’ produced through replication competent mini genome of serotype Asia 1, 63/72, in BHK cell lines

► Here we report the production of chimeric FMDV ‘O’ using Asia 1 replicon. ► The chimeric virus replicated efficiently in BHK 21 cells to produce high titred virus ► The chimeric virus vaccine was found to be potent in vaccinated bovine calves. ► The backbone may be used for developing vaccines wit...

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Bibliographic Details
Published in:Virus research 2013-05, Vol.173 (2), p.299-305
Main Authors: Joshi, Rahul, Chandrasekar, S., Paul, Soumya, Chokkalingam, Ashok Kumar, Saravanan, T., Ahmad, Kakoli, Dechamma, H.J., Reddy, G.R., Gopinath, S.M., Das, D.N., Shaila, M.S., Bharath, G.N., Suryanarayana, V.V.S.
Format: Article
Language:English
Subjects:
Animals
antibodies
Antibodies, Viral - blood
antiserum
Cell Line
Chimeric virus
complementary DNA
Cricetinae
cross reaction
Cross Reactions
FMDV minigenome
Foot-and-mouth disease
Foot-and-mouth disease virus
Foot-and-Mouth Disease Virus - genetics
Foot-and-Mouth Disease Virus - growth & development
Foot-and-Mouth Disease Virus - immunology
genome
pathogenicity
Recombination, Genetic
replicon
serotypes
Serum - immunology
vaccination
Vaccine
Vaccine strain
vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccines, Synthetic - isolation & purification
Viral Plaque Assay
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
Viral Vaccines - isolation & purification
Virus Replication
viruses
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Summary:► Here we report the production of chimeric FMDV ‘O’ using Asia 1 replicon. ► The chimeric virus replicated efficiently in BHK 21 cells to produce high titred virus ► The chimeric virus vaccine was found to be potent in vaccinated bovine calves. ► The backbone may be used for developing vaccines with other strains of interest. Regular vaccinations with potent vaccine, in endemic countries and vaccination to live in non-endemic countries are the methods available to control foot-and-mouth disease. Selection of candidate vaccine strain is not only cumbersome but the candidate should grow well for high potency vaccine preparation. Alternative strategy is to generate an infectious cDNA of a cell culture-adapted virus and use the replicon for development of tailor-made vaccines. We produced a chimeric ‘O’ virus in the backbone of Asia 1 and studied its characteristics. The chimeric virus showed high infectivity titre (>1010) in BHK 21 cell lines, revealed small plaque morphology and there was no cross reactivity with antiserum against Asia 1. The virus multiplies rapidly and reaches peak at 12h post infection. The vaccine prepared with this virus elicited high antibody titres.
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2013.01.018