Expression of type I interferon-induced antiviral state and pro-apoptosis markers during experimental infection with low or high virulence bovine viral diarrhea virus in beef calves

► IFN-α and -β were upregulated 5 days post infection with BVDV-2 but not with BVDV-1. ► BVDV infection (high or low virulence) upregulated type I interferon-induced genes. ► BVDV-1 infection induced higher expression of OAS-1 and ISG-15 than BVDV-2 infection. ► Upregulation of caspase-8 and -9 was...

Full description

Saved in:
Bibliographic Details
Published in:Virus research 2013-05, Vol.173 (2), p.260-269
Main Authors: Palomares, Roberto A., Walz, Heather G., Brock, Kenny V.
Format: Article
Language:English
Subjects:
Animals
Antiviral state
Apoptosis
beef cattle
Bovine viral diarrhea virus
calves
caspase-3
caspase-8
Cattle
Cattle Diseases - immunology
Cattle Diseases - pathology
cell culture
culture media
Cytokines
Diarrhea Virus 1, Bovine Viral - immunology
Diarrhea Virus 1, Bovine Viral - pathogenicity
Diarrhea Virus 2, Bovine Viral - immunology
Diarrhea Virus 2, Bovine Viral - pathogenicity
Disease Models, Animal
Gene Expression
Gene Expression Profiling
gene expression regulation
genes
genotype
Host-Pathogen Interactions
Interferon
Interferon Type I - biosynthesis
Interferon Type I - genetics
interferon-alpha
interferon-beta
lymph nodes
Lymph Nodes - pathology
Lymph Nodes - virology
messenger RNA
Pestivirus Infections - immunology
Pestivirus Infections - pathology
Real-Time Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Signal Transduction
spleen
Spleen - pathology
Spleen - virology
virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► IFN-α and -β were upregulated 5 days post infection with BVDV-2 but not with BVDV-1. ► BVDV infection (high or low virulence) upregulated type I interferon-induced genes. ► BVDV-1 infection induced higher expression of OAS-1 and ISG-15 than BVDV-2 infection. ► Upregulation of caspase-8 and -9 was only observed in calves infected with BVDV-1. The objective of this study was to compare the mRNA expression of host genes involved in type-I interferon-induced antiviral state (IFN-α, IFN-β, Mx-1, PKR, OAS-1 and ISG-15), and apoptosis (caspase-3, -8, and -9), after experimental infection of beef calves with low or high virulence noncytopathic (ncp) bovine viral diarrhea virus (BVDV) strains. Thirty BVDV-naïve, clinically normal calves were randomly assigned to three groups. Calves were intranasally inoculated with low (LV; n=10, strain SD-1) or high (HV; n=10, strain 1373) virulence ncp BVDV or BVDV-free cell culture medium (Control, n=10). Quantitative RT-PCR was used to determine the target gene expression in tracheo-bronchial lymph nodes and spleen 5 days after infection. Interferon-α and -β mRNA levels were up-regulated in tracheo-bronchial lymph nodes (P
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2013.02.010