Molecular epidemiology of extended-spectrum β-lactamase-, AmpC β-lactamase- and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae isolated from Canadian hospitals over a 5 year period: CANWARD 2007-11

To assess the proportion of Escherichia coli and Klebsiella pneumoniae from Canadian hospitals that produce extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases and carbapenemases, as well as to describe the patterns of antibiotic resistance and molecular characteristics of these organisms. Som...

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Published in:	Journal of antimicrobial chemotherapy 2013-05, Vol.68 Suppl 1, p.i57-i65
Main Authors:	Denisuik, Andrew J, Lagacé-Wiens, Philippe R S, Pitout, Johann D, Mulvey, Michael R, Simner, Patricia J, Tailor, Franil, Karlowsky, James A, Hoban, Daryl J, Adam, Heather J, Zhanel, George G
Format:	Article
Language:	eng
Subjects:	Adolescent Adult Aged Anti-Bacterial Agents - pharmacology Bacteria - classification Bacteria - drug effects Bacteria - genetics Bacteria - isolation & purification Bacterial Infections - epidemiology Bacterial Infections - microbiology Bacterial Proteins - genetics beta-Lactam Resistance - genetics beta-Lactamases - genetics Canada - epidemiology Child Cross Infection - epidemiology Cross Infection - history Drug Resistance, Multiple, Bacterial - genetics Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - history Female Genotype History, 21st Century Humans Klebsiella Infections - epidemiology Klebsiella Infections - history Klebsiella pneumoniae - genetics Klebsiella pneumoniae - isolation & purification Male Microbial Sensitivity Tests Middle Aged Molecular Sequence Data Young Adult
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Summary:	To assess the proportion of Escherichia coli and Klebsiella pneumoniae from Canadian hospitals that produce extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases and carbapenemases, as well as to describe the patterns of antibiotic resistance and molecular characteristics of these organisms. Some 5451 E. coli and 1659 K. pneumoniae were collected from 2007 to 2011 inclusive as part of the ongoing CANWARD national surveillance study. Antimicrobial susceptibility testing was performed to detect putative ESBL, AmpC and carbapenemase producers, which were then further characterized by PCR and sequencing to detect resistance genes. In addition, isolates were characterized by PFGE and an allele-specific PCR to detect isolates of sequence type (ST) 131. The proportion of ESBL-producing E. coli (2007, 3.4%; 2011, 7.1%), AmpC-producing E. coli (2007, 0.7%; 2011, 2.9%) and ESBL-producing K. pneumoniae (2007, 1.5%; 2011, 4.0%) among the isolates collected increased during the study period. The majority of ESBL-producing E. coli (>95%), AmpC-producing E. coli (>97%) and ESBL-producing K. pneumoniae (>89%) remained susceptible to colistin, amikacin, ertapenem and meropenem. Isolates were generally unrelated by PFGE (
ISSN:	1460-2091