Ultraviolet-radiation-resistant isolates revealed cellulose-degrading species of Cellulosimicrobium cellulans (UVP1) and Bacillus pumilus (UVP4)

Among extremophiles, microorganisms resistant to ultraviolet radiation (UVR) have been known to produce a variety of metabolites (i.e., extremolytes). We hypothesized that natural microbial flora on elevated land (hills) would reveal a variety of UVR‐resistant extremophiles and polyextremophiles wit...

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Published in:Biotechnology and applied biochemistry 2012-09, Vol.59 (5), p.395-404
Main Authors: Gabani, Prashant, Copeland, Erin, Chandel, Anuj K., Singh, Om V.
Format: Article
Language:English
Subjects:
Actinomycetales - genetics
Actinomycetales - isolation & purification
Actinomycetales - metabolism
Actinomycetales - radiation effects
Agar
Bacillus
Bacillus - genetics
Bacillus - isolation & purification
Bacillus - metabolism
Bacillus - radiation effects
bioenergy
Biological and medical sciences
Biotechnology
Cellulose - metabolism
Elevated
Enzymes
extremophiles
Fundamental and applied biological sciences. Psychology
Hydrolysis - radiation effects
Land
Microorganisms
polyextremophiles
Proteins
Proteome - metabolism
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sequencing
therapeutics
ultraviolet radiation
Ultraviolet Rays
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Summary:Among extremophiles, microorganisms resistant to ultraviolet radiation (UVR) have been known to produce a variety of metabolites (i.e., extremolytes). We hypothesized that natural microbial flora on elevated land (hills) would reveal a variety of UVR‐resistant extremophiles and polyextremophiles with modulated proteins and enzymes that had biotechnological implications. Microorganisms Cellulosimicrobium cellulans UVP1 and Bacillus pumilus UVP4 were isolated and identified using 16S rRNA sequencing, and showed extreme UV resistance (1.03 × 106 and 1.71 × 105 J/m2, respectively) from elevated land soil samples along with unique patterns of protein expression under UVR and non‐UVR. A broad range of cellulolytic activity on carboxymethyl cellulose agar plates in C. cellulans UVP1 and B. pumilus UVP4 was revealed at varying pH, temperature, and inorganic salt concentration. Further, the microbial strain B. pumilus UVP4 showed the basic characteristics of a novel group: polyextremophiles with significance in bioenergy.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1038