A comparison of tissue-engineered bone from adipose-derived stem cell with autogenous bone repair in maxillary alveolar cleft model in dogs

Abstract This study was designed to compare bone regeneration of tissue-engineered bone from adipose-derived stem cell and autogenous bone graft in a canine maxillary alveolar cleft model. In this prospective clinical trial, mesenchymal stem cells (MSCs) were isolated from subcutaneous canine adipos...

Full description

Saved in:
Bibliographic Details
Published in:International journal of oral and maxillofacial surgery 2013-05, Vol.42 (5), p.562-568
Main Authors: Pourebrahim, N, Hashemibeni, B, Shahnaseri, S, Torabinia, N, Mousavi, B, Adibi, S, Heidari, F, Alavi, M. Jafary
Format: Article
Language:English
Subjects:
adipose-derived stem cell (ADSC)
alveolar cleft
Alveolectomy - methods
Animals
Bone Marrow - pathology
Bone Regeneration - physiology
Bone Transplantation - methods
Bone Transplantation - pathology
Calcification, Physiologic - physiology
Cell Culture Techniques
Cell Differentiation - physiology
Cell Separation
Cleft Palate - surgery
Collagen - analysis
Culture Media
Dentistry
Disease Models, Animal
Dogs
Durapatite - chemistry
Flow Cytometry
HA/TCP
histomorphometry
Maxilla - surgery
Mesenchymal Stromal Cells - physiology
Microscopy, Electron, Scanning
Osteoblasts - pathology
Osteogenesis - physiology
Prospective Studies
Subcutaneous Fat - cytology
Surgery
Tibia - surgery
Time Factors
Tissue Engineering - methods
tissue engineering of bone
Tissue Scaffolds - chemistry
Transplant Donor Site - surgery
Transplantation, Autologous
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract This study was designed to compare bone regeneration of tissue-engineered bone from adipose-derived stem cell and autogenous bone graft in a canine maxillary alveolar cleft model. In this prospective clinical trial, mesenchymal stem cells (MSCs) were isolated from subcutaneous canine adipose tissue. Undifferentiated cells were incubated with a 3 mm × 3 mm × 3 mm hydroxyapatite/beta-tricalcium phosphate scaffold, in specific osteogenic medium for 21 days. Four mongrel dogs were prepared by removal of two of the three incisors bilaterally and a 15 mm defect in bone was created from crest to nasal floor. After healing, repair was followed by a tissue engineered bone graft from adipose-derived stem cells on one side and corticocancellous tibial auto graft on the other side. Bone regeneration was evaluated by histomorphometry on days 15 and 60 after implantation. The data were analysed with descriptive and t test methods ( α = 0.05). Bone formation on the autograft sides was higher than on the stem cell sides at 15 and 60 days, 45% and 96% versus 5% and 70%, respectively. Differences between the two groups at 15 and 60 days were significant ( p = 0.004 and 0.001, respectively). Although autograft is still the gold standard for bone regeneration, tissue engineered bone may provide an acceptable alternative.
ISSN:0901-5027
1399-0020
DOI:10.1016/j.ijom.2012.10.012