Characterization of cytotoxicity-related gene expression in response to virulent Marek’s disease virus infection in the bursa of Fabricius

Cell-mediated cytotoxic responses are critical for control of Marek’s disease virus (MDV) infection and tumour development. However, the mechanisms of virus clearance mediated by cytotoxic responses in the bursa of Fabricius of chickens during MDV infection are not fully understood. In this study, t...

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Bibliographic Details
Published in:Research in veterinary science 2013-06, Vol.94 (3), p.496-503
Main Authors: Chen, Cui-Ying, Xie, Qing-Mei, Xue, Yu, Ji, Jun, Chang, Shuang, Ma, Jing-Yun, Bi, Ying-Zuo
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - immunology
Blotting, Western - veterinary
bursa of Fabricius
Bursa of Fabricius - immunology
Bursa of Fabricius - metabolism
Bursa of Fabricius - physiopathology
Cells
chickens
Chickens - immunology
Chickens - metabolism
Cytotoxic responses
cytotoxicity
DNA repair
DNA Repair - immunology
Fas Ligand Protein - metabolism
fas Receptor - metabolism
gene expression
Gene Expression Regulation
genes
Herpesvirus 3, Gallid - immunology
Herpesvirus 3, Gallid - physiology
Immune system
Immunity, Cellular - immunology
Immunity, Cellular - physiology
Immunology
Marek Disease - immunology
Marek Disease - metabolism
Marek’s disease virus
Polymerase Chain Reaction - veterinary
Poultry
Proteins
Real-Time Polymerase Chain Reaction - veterinary
The bursa of Fabricius
Veterinary medicine
Virus Replication
viruses
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Summary:Cell-mediated cytotoxic responses are critical for control of Marek’s disease virus (MDV) infection and tumour development. However, the mechanisms of virus clearance mediated by cytotoxic responses in the bursa of Fabricius of chickens during MDV infection are not fully understood. In this study, the host cytotoxic responses during MDV infection in the bursa were investigated by examining the expression of genes in the cell lysis pathways. Partial up-regulation existed in the expression of the important cytolytic molecule granzyme A (GzmA), Fas, NK lysin and DNA repair enzyme Ape1, whereas little or no expression appeared in other cytolytic molecules, including perforin (PFN) and Fas ligand (FasL), and molecules involved in DNA repair and apoptosis in the bursa during MDV infection. These results suggest that less sustained cytotoxic activities are generated in the bursa of MDV-infected chickens. The findings of this study provide a more detailed insight into the host cytotoxic responses to MDV infection.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2012.10.014