Expression profiles of subtracted mRNAs during cellular senescence in human mesenchymal stem cells derived from bone marrow

Cellular senescence is an irreversible cell cycle arrest that limits the replicative lifespan of cells. Senescence suppresses development of tumors by regulating aging factors, such as cyclin dependent kinase inhibitor (CKI) and telomerase. Suppression subtractive hybridization (SSH) was used to ide...

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Bibliographic Details
Published in:Experimental gerontology 2013-05, Vol.48 (5), p.464-471
Main Authors: Yoo, Jung Ki, Choi, Seong-jun, Kim, Jin Kyeoung
Format: Article
Language:English
Subjects:
Bone Marrow Cells - metabolism
Bone Marrow Cells - physiology
Cell Adhesion - genetics
Cell Adhesion - physiology
Cells, Cultured
Cellular Senescence - genetics
Cellular Senescence - physiology
Culture Media, Serum-Free
Differentially expressed genes
Gene Expression Profiling - methods
Gene Expression Regulation - physiology
Humans
Mesenchymal stem cells
Mesenchymal Stromal Cells - metabolism
Mesenchymal Stromal Cells - physiology
RNA, Messenger - genetics
RT-PCR
Senescence
Suppression subtractive hybridization
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Summary:Cellular senescence is an irreversible cell cycle arrest that limits the replicative lifespan of cells. Senescence suppresses development of tumors by regulating aging factors, such as cyclin dependent kinase inhibitor (CKI) and telomerase. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed between young human mesenchymal stem cells (Y-hMSCs) and senescent human mesenchymal stem cells (S-hMSCs). We selected positive clones that were functionally characterized by referring to public databases using NCBI BLAST tool. This search revealed that 19 genes were downregulated, and 43 genes were upregulated in S-hMSCs relative to Y-hMSCs. Among subtracted clones in Y-hMSCs, most of genes markedly were related to metabolic functions. These genes, PDIA3, WDR1, FSTL1, COPG1, LMAN1, and PDIA6, significantly downregulated. Conversely, genes for subtracted clones in S-hMSCs were mostly associated with cell adhesion. In particular, the expression levels of 9 genes, HSP90B1, EID1, ATP2B4, DDAH1, PRNP, RAB1A, PGS5, TM4SF1 and SSR3, gradually increased during senescence. These genes have not previously been identified as being related to cellular senescence, but they seemed to be potentially affected during cellular senescence.
ISSN:0531-5565
1873-6815
DOI:10.1016/j.exger.2013.02.022