Radiorespirometric assays for the detection of biogenic sulfides from sulfate‐reducing bacteria

Aims The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H2S in trace concentrations, to correlate with sulfate‐reducing bacterial activity. Methods and Results Ser...

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Bibliographic Details
Published in:Journal of applied microbiology 2013-04, Vol.114 (4), p.1008-1019
Main Authors: Queiroz, J.C., Melo Ferreira, A.C., Costa, A.C.A.
Format: Article
Language:English
Subjects:
Bacteria
Bioassays
Biological and medical sciences
Carbon - metabolism
Corrosion
culture medium
Desulfovibrio
Desulfovibrio - metabolism
Fundamental and applied biological sciences. Psychology
Microbiology
most probable number
Offshore oil wells
radiorespirometry
Seawater - microbiology
Sulfates - metabolism
sulfate‐reducing bacteria
Sulfides - isolation & purification
Sulfur-Reducing Bacteria - metabolism
sulphide
Water Microbiology
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Summary:Aims The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H2S in trace concentrations, to correlate with sulfate‐reducing bacterial activity. Methods and Results Serial dilutions of synthetic sea water with a pure culture of Desulfovibrio alaskensis, a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction. Conclusions Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum. Significance and Impact of the Study The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12122