Comparison of two selective media for the recovery of Clostridium difficile from environmental surfaces

Summary Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin–cycloserine–egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile . A wide range of environmental surfaces was sampled using ster...

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Bibliographic Details
Published in:The Journal of hospital infection 2013-02, Vol.83 (2), p.164-166
Main Authors: Hill, K.A, Collins, J, Wilson, L, Perry, J.D, Gould, F.K
Format: Article
Language:English
Subjects:
Agar
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Bacteriological Techniques - methods
Biological and medical sciences
C. difficile infection
Clostridium difficile
Clostridium difficile - isolation & purification
Culture media
Culture Media - chemistry
Environmental Microbiology
Environmental surfaces
Gastroenterology. Liver. Pancreas. Abdomen
Hospitals
Human bacterial diseases
Infectious Disease
Infectious diseases
Medical sciences
Other diseases. Semiology
Sensitivity and Specificity
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
United Kingdom
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Summary:Summary Two culture media were compared for their ability to isolate Clostridium difficile from environmental sites within a UK hospital. The media were cefoxitin–cycloserine–egg yolk agar plus lysozyme (CCEY/L) and chromID C. difficile . A wide range of environmental surfaces was sampled using sterile sponges (Polywipes) and these were inoculated on to both media. C. difficile was recovered from 105 of 496 sites (21%) using a combination of both media. The sensitivity of chromID C. difficile was 87.6% compared with 26.6% for CCEY/L ( P  
ISSN:0195-6701
1532-2939
DOI:10.1016/j.jhin.2012.10.006