Assessing the contribution of HRPT2 to the pathogenesis of jaw fibrous dysplasia, ossifying fibroma, and osteosarcoma

Objective To investigate HRPT2 in jaw ossifying fibroma (OF), fibrous dysplasia (FD), and osteosarcoma (OS). Study Design We combined microsatellite loss of heterozygosity (LOH), HRPT2 sequence alterations at the mRNA level by reverse-transcription polymerase chain reaction (PCR), cDNA sequencing, a...

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Published in:Oral surgery, oral medicine, oral pathology and oral radiology oral medicine, oral pathology and oral radiology, 2013-03, Vol.115 (3), p.359-367
Main Authors: de Mesquita Netto, Ana Carolina, DDS, PhD, Gomez, Ricardo Santiago, DDS, PhD, Diniz, Marina Gonçalves, DDS, PhD, Fonseca-Silva, Thiago, DDS, MSc, Campos, Kelma, DDS, MSc, De Marco, Luiz, MD, PhD, Carlos, Román, DDS, Gomes, Carolina Cavaliéri, DDS, PhD
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Chromosome Mapping
Chromosomes, Human, Pair 1 - genetics
Cyclin D1 - genetics
Dentistry
Disease Progression
Exons - genetics
Female
Fibroma, Ossifying - genetics
Fibrous Dysplasia of Bone - genetics
Gene Silencing
Humans
Hyperparathyroidism - genetics
Jaw Diseases - genetics
Jaw Neoplasms - genetics
Loss of Heterozygosity - genetics
Male
Microsatellite Repeats - genetics
Middle Aged
Mutation - genetics
Osteosarcoma - genetics
RNA, Messenger - genetics
Sequence Deletion - genetics
Surgery
Transcription, Genetic - genetics
Tumor Suppressor Proteins - genetics
Young Adult
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Summary:Objective To investigate HRPT2 in jaw ossifying fibroma (OF), fibrous dysplasia (FD), and osteosarcoma (OS). Study Design We combined microsatellite loss of heterozygosity (LOH), HRPT2 sequence alterations at the mRNA level by reverse-transcription polymerase chain reaction (PCR), cDNA sequencing, and quantitative PCR (qPCR) and immunohistochemistry (IHC) in a total of 19 OF, 15 FD, and 9 OS. Because HRPT2 (parafibromin) interacts with cyclin D1, we investigated cyclin D1 expression with the use of qPCR and IHC. Results LOH was detected in 3/5 FD, 6/9 OF, and 2/2 OS heterozygous samples. LOH was not associated with decreased mRNA levels or HRPT2 protein expression except for 1 OF which harbored an inactivating mutation. However, this tumor did not display altered transcription or protein levels of HRPT2 nor cyclin compared with the other OF. Conclusions The contribution of HRPT2 inactivation to the pathogenesis of OF, FD, and OS is marginal at best and may be limited to progression rather than tumor initiation.
ISSN:2212-4403
2212-4411
DOI:10.1016/j.oooo.2012.11.015