A comparison of Api 20A vs MALDI-TOF MS for routine identification of clinically significant anaerobic bacterial strains to the species level

Adequate identification of anaerobic bacteria still presents a challenge for laboratories conducting microbiological diagnostics. The aim of this study was to compare the use of Api 20A and MALDI-TOF MS techniques for identification of obligate anaerobes. The results indicate that MALDI-TOF MS ensur...

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Bibliographic Details
Published in:Journal of microbiological methods 2013-02, Vol.92 (2), p.209-212
Main Authors: Kierzkowska, Marta, Majewska, Anna, Kuthan, Robert T., Sawicka-Grzelak, Anna, Młynarczyk, Grażyna
Format: Article
Language:English
Subjects:
Anaerobes
Bacteria
Bacteria, Anaerobic - chemistry
Bacteria, Anaerobic - classification
Bacteria, Anaerobic - isolation & purification
Bacteria, Anaerobic - metabolism
Bacterial Infections - diagnosis
Bacterial Typing Techniques - methods
diagnostic techniques
Humans
MALDI-TOF MS
matrix-assisted laser desorption-ionization mass spectrometry
patients
Phenotypic identification
species identification
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
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Summary:Adequate identification of anaerobic bacteria still presents a challenge for laboratories conducting microbiological diagnostics. The aim of this study was to compare the use of Api 20A and MALDI-TOF MS techniques for identification of obligate anaerobes. The results indicate that MALDI-TOF MS ensures a rapid and accurate identification of the species isolated from patients.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2012.12.007