A Locked Nucleic Acid-Based Nanocrawler: Designed and Reversible Movement Detected by Multicolor Fluorescence

Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2′-amin...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2013-02, Vol.135 (7), p.2423-2426
Main Authors: Astakhova, I. Kira, Pasternak, Karol, Campbell, Meghan A, Gupta, Pankaj, Wengel, Jesper
Format: Article
Language:English
Subjects:
Color
Fluorescence
Molecular Structure
Oligonucleotides - chemistry
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Summary:Herein we introduce a novel fluorescent LNA/DNA machine, a nanocrawler, which reversibly moves along a directionally polar complementary road controlled by affinity-enhancing locked nucleic acid (LNA) monomers and additional regulatory strands. Polyaromatic hydrocarbon (PAH) dyes attached to 2′-amino-LNA monomers are incorporated at four stations of the system, enabling simple detection of the position of the nanocrawler via a step-specific color signal. The sensing is provided by highly sensitive, chemically stable, and photostable PAH LNA interstrand communication systems, including pyrene excimer formation and pyrene–perylene interstrand Förster resonance energy transfer. We furthermore demonstrate that the nanocrawler selectively and reversibly moves along the road, followed by a bright and consistent fluorescence response for up to 10 cycles without any loss of signal.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja311250w