Anti-inflammatory intestinal activity of Arctium lappa L. (Asteraceae) in TNBS colitis model

In Brazilian traditional medicine, Arctium lappa (Asteraceae), has been reported to relieve gastrointestinal symptoms. In the present study, we investigated the effects of the lactone sesquiterpene onopordopicrin enriched fraction (ONP fraction) from Arctium lappa in an experimental colitis model in...

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Published in:Journal of ethnopharmacology 2013-03, Vol.146 (1), p.300-310
Main Authors: de Almeida, Ana Beatriz Albino, Sánchez-Hidalgo, Marina, Martín, Antonio Ramón, Luiz-Ferreira, Anderson, Trigo, José Roberto, Vilegas, Wagner, dos Santos, Lourdes Campaner, Souza-Brito, Alba Regina Monteiro, de la Lastra, Catalina Alarcón
Format: Article
Language:English
Subjects:
acute effects
Animals
Anti-Inflammatory Agents - therapeutic use
Arctium
Arctium lappa
Colitis
Colitis - chemically induced
Colitis - drug therapy
Colitis - metabolism
Colitis - pathology
COX-2
Cyclooxygenase 2 - metabolism
Disease Models, Animal
edema
gastrointestinal system
immunohistochemistry
inflammation
Male
mucus
myeloperoxidase
necrosis
Onopordopicrin
oral administration
Peroxidase - metabolism
Phytotherapy
Plant Extracts - therapeutic use
Plant Leaves
prostaglandin synthase
protein synthesis
Rats
Rats, Wistar
secretion
sulfonic acid
TNBS
traditional medicine
Trinitrobenzenesulfonic Acid
tumor necrosis factor-alpha
Tumor Necrosis Factor-alpha - metabolism
Western blotting
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Summary:In Brazilian traditional medicine, Arctium lappa (Asteraceae), has been reported to relieve gastrointestinal symptoms. In the present study, we investigated the effects of the lactone sesquiterpene onopordopicrin enriched fraction (ONP fraction) from Arctium lappa in an experimental colitis model induced by 2,4,6 trinitrobenzene sulfonic acid and performed experiments to elucidate the underlying action mechanisms involved in that effect. ONP fraction (25 and 50mg/kg/day) was orally administered 48, 24 and 1h prior to the induction of colitis and 24h after. The inflammatory response was assessed by gross appearance, myeloperoxidase (MPO) activity, tumor necrosis factor alpha (TNF-α) levels and a histological study of the lesions. We determined cyclooxygenase (COX)-1 and -2 protein expressions by western blotting and immunohistochemistry assays. TNBS group was characterized by increased colonic wall thickness, edema, diffuse inflammatory cell infiltration, increased MPO activity and TNF-α levels. On the contrary, ONP fraction (25 and 50mg/kg) treatment significantly reduced the macroscopic inflammation scores (p
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2012.12.048