Effects of xenogeneic adipose-derived stem cell transplantation on acute-on-chronic liver failure

BACKGROUND:Adipose-derived stem cells(ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure(ACLF) This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.METHODS:ADSCs isolated from porcine fat tissue...

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Published in:Hepatobiliary & pancreatic diseases international 2013-02, Vol.12 (1), p.60-67
Main Authors: Zhu, Wei, Shi, Xiao-Lei, Xiao, Jiang-Qiang, Gu, Guang-Xiang, Ding, Yi-Tao, Ma, Zheng-Liang
Format: Article
Language:English
Subjects:
acute-on-chronic
acute-on-chronic liver failure
Adipose Tissue - cytology
Adipose Tissue - metabolism
adipose-derived
adipose-derived stem cells
Animals
Animals, Outbred Strains
Biomarkers - metabolism
cell
Cell Differentiation
cell transplantation
cells
Disease Models, Animal
End Stage Liver Disease - mortality
End Stage Liver Disease - pathology
End Stage Liver Disease - therapy
Endocrinology & Metabolism
failure
Gastroenterology and Hepatology
Graft Survival
Hepatocytes - cytology
Hepatocytes - metabolism
liver
Liver Failure, Acute - mortality
Liver Failure, Acute - pathology
Liver Failure, Acute - therapy
Liver Function Tests
Male
Rabbits
stem
Stem Cell Transplantation - methods
Stem Cell Transplantation - mortality
Stem Cells - cytology
Stem Cells - metabolism
Survival Rate
Swine
transplantation
Transplantation, Heterologous
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Summary:BACKGROUND:Adipose-derived stem cells(ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure(ACLF) This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.METHODS:ADSCs isolated from porcine fat tissue were expanded and labeled with BrdU.Rabbit models of ACLF were created by administration of D-Gal following CCl 4-induced cirrhosis.One day after administration of D-Gal,rabbits of the ACLF/ADSCs group(n=15) were received ADSCs transplantation,while those in the ACLF/saline group(n=15) were treated with the same volume of saline.Biochemical parameters and histomorphological scoring were evaluated;the distribution and characteristics of transplanted ADSCs as well as the pathology of the liver were examined.RESULTS:ADSCs transplantation improved the survival rate and the liver function of rabbits with ACLF.Biochemical parameters of the ACLF/ADSCs group were improved compared with those of the ACLF/saline group,and histomorphological scoring of the ACLF/ADSCs group was significantly lower than that of the ACLF/saline group.ADSCs were identified in the periportal region of the liver after cell transplantation.CONCLUSION:Xenogenic ADSCs have therapeutic efficacy in the ACLF rabbit model.
ISSN:1499-3872
DOI:10.1016/S1499-3872(13)60007-7