Mechanism of regulating the expression of λN gene by ribosomal protein at translational level

In ribosomal protein S12 mutant or L24 mutant the expression of λN gene was depressed at translational level. To study its mechanism the λN gene region of λN -lacZ gene fusion was trimmed from its 5′ end to 3′ end with DNA exonuclease III (DNA cxoIII) in order to alter the TIR (translational initiat...

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Bibliographic Details
Published in:Science China. Life sciences 1998-02, Vol.41 (1), p.29-36
Main Authors: Li, Muyang, Weng, Manli, Tong, Kezhong
Format: Article
Language:English
Subjects:
DNA sequencing
Exonuclease
Gene expression
Gene fusion
Initiation complex
Kinases
LacZ gene
Mutants
Proteins
Ribosomal protein S12
Translation
Translation initiation
β-Galactosidase
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Summary:In ribosomal protein S12 mutant or L24 mutant the expression of λN gene was depressed at translational level. To study its mechanism the λN gene region of λN -lacZ gene fusion was trimmed from its 5′ end to 3′ end with DNA exonuclease III (DNA cxoIII) in order to alter the TIR (translational initiation region) and the ding region of λN gene. After DNA sequencing 23 species of different λN-lacZ fused genes were obtained. The β-galactosidase activities of these deletants in ribosomal protein mutant were compared with that in wild type strain. The result indicated that (i) S12 mutant could affect 305 subunit’s binding to the TIR of λN gene messenger and cause the difficulty in forming 30s initiation complex and then decrease the efficiency of translational initiation; (ii) in S12 mutant the coding region of λN gene alw affected the expression λN gene; (iii) in L24 mutant the inhibition of λN gene expression was not related to translational initiation and the 5′ end of the coding region of λN gene, but related to the 3′ end of λN gene.
ISSN:1006-9305
1674-7305
1862-2798
1869-1889
DOI:10.1007/BF02882703