Differentiation of mouse induced pluripotent stem cells into corneal epithelial-like cells

Somatic cells can be reprogrammed into a pluripotent ES‐cell‐like state (termed induced pluripotent stem cells, iPS) by transcription factors, which have enormous therapeutic potential for regenerative medicine. We have investigated whether iPS can directly differentiate into corneal epithelium‐like...

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Bibliographic Details
Published in:Cell biology international 2013-01, Vol.37 (1), p.87-94
Main Authors: Yu, Dan, Chen, Mengfei, Sun, Xuerong, Ge, Jian
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Coculture Techniques
corneal epithelial cells
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelium, Corneal - cytology
Epithelium, Corneal - metabolism
induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mice
microenvironment
Microscopy, Electron, Scanning
Real-Time Polymerase Chain Reaction
reconstruction
reprogramming
Transcription Factors - metabolism
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Summary:Somatic cells can be reprogrammed into a pluripotent ES‐cell‐like state (termed induced pluripotent stem cells, iPS) by transcription factors, which have enormous therapeutic potential for regenerative medicine. We have investigated whether iPS can directly differentiate into corneal epithelium‐like cells. Mouse iPS cells were co‐cultured with corneal limbal stroma. RT‐PCR, immunohistochemistry and scanning electron microscopy analysis were used to detect differentiated iPS. Undifferentiated iPS cells expressed ES cells related genes. Co‐culture with corneal limbal stroma, in the presence of additional factors bFGF, EGF and NGF, activated keratin expression 12 (K12, a marker of corneal epithelial cells) and downregulated Nanog. These data suggest that mouse iPS cells can differentiate into corneal epithelial‐like cells by replication of a corneal epithelial stem cell niche.
ISSN:1065-6995
1095-8355
DOI:10.1002/cbin.10007