Resveratrol decreases inflammation and increases utrophin gene expression in the mdx mouse model of duchenne muscular dystrophy

Summary Background & aims Duchenne muscular dystrophy (DMD) is a lethal genetic disease with no cure. Reducing inflammation or increasing utrophin expression can alleviate DMD pathology. Resveratrol can reduce inflammation and activate the utrophin promoter. The aims of this study were to identi...

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Bibliographic Details
Published in:Clinical nutrition (Edinburgh, Scotland) Scotland), 2013-02, Vol.32 (1), p.104-111
Main Authors: Gordon, Bradley S, Delgado Díaz, Diana C, Kostek, Matthew C
Format: Article
Language:English
Subjects:
animal models
Animals
Anti-Inflammatory Agents, Non-Steroidal - administration & dosage
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Biological and medical sciences
Body Weight
Dietary Supplements
Disease Models, Animal
Diseases of striated muscles. Neuromuscular diseases
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Gastroenterology and Hepatology
gene expression
immunohistochemistry
Inflammation
Inflammation Mediators - metabolism
interleukin-6
Leukocytes - immunology
Leukocytes - metabolism
Leukocytes - pathology
macrophages
Macrophages - immunology
Macrophages - metabolism
Macrophages - pathology
Male
Medical sciences
messenger RNA
Mice
Mice, Inbred mdx
Muscle Development
Muscle, Skeletal - immunology
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
muscles
muscular dystrophy
Muscular Dystrophy, Duchenne - diet therapy
Muscular Dystrophy, Duchenne - immunology
Muscular Dystrophy, Duchenne - metabolism
Muscular Dystrophy, Duchenne - pathology
Neurology
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
PGC-1α
protein synthesis
Resveratrol
reverse transcriptase polymerase chain reaction
RNA, Messenger - metabolism
Sirtuin 1 - biosynthesis
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
Stilbenes - administration & dosage
Stilbenes - therapeutic use
therapeutics
Trans-Activators - biosynthesis
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription Factors
tumor necrosis factor-alpha
Up-Regulation
Utrophin
Utrophin - biosynthesis
Utrophin - genetics
Utrophin - metabolism
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Western blotting
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Summary:Summary Background & aims Duchenne muscular dystrophy (DMD) is a lethal genetic disease with no cure. Reducing inflammation or increasing utrophin expression can alleviate DMD pathology. Resveratrol can reduce inflammation and activate the utrophin promoter. The aims of this study were to identify an active dose of resveratrol in mdx mice and examine if this dose decreased inflammation and increased utrophin expression. Methods 5-week old mdx mice were given 0, 10, 100, or 500 mg/kg of resveratrol everyday for 10 days. Sirt1 was measured by qRT-PCR and used to determine the most active dose. Muscle inflammation was measured by H&E staining, CD45 and F4/80 immunohistochemistry. IL-6, TNFα, PGC-1α, and utrophin gene expression were measured by qRT-PCR. Utrophin, Sirt1, and PGC-1α protein were quantified by western blot. Results The 100 mg/kg dose of resveratrol, the most active dose, increased Sirt1 mRNA 60 ± 10% ( p  
ISSN:0261-5614
1532-1983
DOI:10.1016/j.clnu.2012.06.003