In vitro Survival of Follicles Collected from Domestic Cats' Ovaries at Different Stages of Oestrous Cycle and Cultured with IGF-1

Contents Optimal conditions for in vitro culture of feline ovarian follicles have not yet been defined. Follicular development is regulated by intraovarian growth factors, as insulin‐like growth factor (IGF‐1), and during the different stages of the oestrous cycle, follicles are exposed to specific...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-12, Vol.47 (s6), p.109-112
Main Authors: Alves, EA, Padilha, L, Savi, PA, Apparicio, MF, Mostachio, GQ, Motheo, TF, Pires-Buttler, EA, Vicente, WRR, Luvoni, GC
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Cats
Cats - physiology
Cell culture
Culture Media - chemistry
Estrous Cycle - physiology
Female
Insulin-Like Growth Factor I - pharmacology
Ovarian Follicle - drug effects
Ovarian Follicle - physiology
Tissue Culture Techniques - veterinary
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Summary:Contents Optimal conditions for in vitro culture of feline ovarian follicles have not yet been defined. Follicular development is regulated by intraovarian growth factors, as insulin‐like growth factor (IGF‐1), and during the different stages of the oestrous cycle, follicles are exposed to specific hormonal environments. The aim of this study was to investigate the effect of IGF‐1 on in vitro growth and granulosa cell (GC) viability of preantral follicles collected from domestic cats at follicular and luteal phases of the oestrous cycle. Oestrus and ovulation were induced in 12 cats. A total of 39 and 32 follicles collected at the follicular and luteal phases, respectively, were individually cultured in vitro for 6 days in minimum essential medium media supplemented with or without IGF‐1 (100 ng/ml). Follicles collected during the follicular phase and cultured without IGF‐1 displayed a significant increase in size and higher GC viability (46.5 ± 22.1 μm, 66.7%, respectively) than that of follicles collected at the luteal phase and cultured without IGF‐1 (26.7 ± 14.4 μm, 50%, respectively; p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12001