Genetic deletion of HRP2 and HRP3 in Indian Plasmodium falciparum population and false negative malaria rapid diagnostic test

We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes. Lack of amplification of Pfhrp2, Pfhrp3 and their flanking genes in two P. falciparum field isolates of India. Row 1: Lanes 1 and 7=100bp marker, Lanes 6 and 12=negative control (distilled water), Lanes 2–5=P...

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Bibliographic Details
Published in:Acta tropica 2013-01, Vol.125 (1), p.119-121
Main Authors: Kumar, Navin, Pande, Veena, Bhatt, R.M., Shah, Naman K., Mishra, Neelima, Srivastava, Bina, Valecha, Neena, Anvikar, Anupkumar R.
Format: Article
Language:English
Subjects:
Antigens, Protozoan - analysis
Antigens, Protozoan - genetics
Diagnostic Tests, Routine
False Negative Reactions
Gene Deletion
Genetic deletion
Humans
Immunoassay - methods
India
Malaria
Malaria, Falciparum - diagnosis
Malaria, Falciparum - parasitology
Parasitology - methods
Pfhrp2
Pfhrp3
Plasmodium falciparum
Plasmodium falciparum - immunology
Plasmodium falciparum - isolation & purification
Protozoan Proteins - analysis
Protozoan Proteins - genetics
Rapid diagnostic tests
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Summary:We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes. Lack of amplification of Pfhrp2, Pfhrp3 and their flanking genes in two P. falciparum field isolates of India. Row 1: Lanes 1 and 7=100bp marker, Lanes 6 and 12=negative control (distilled water), Lanes 2–5=Pfhrp2 gene, Lanes 8–11=Pfhrp3 gene. Row 2: Lanes 1 and 7=100bp marker, Lanes 6 and 12=negative control (distilled water), Lanes 2–5=upstream Pfhrp2 gene, Lanes 8–11=downstream Pfhrp2 gene. [Display omitted] ► Failure to express Pfhrp2 and Pfhrp3 due to gene deletion may contribute to variable performance of malaria RDTs. ► We report two Indian P. falciparum isolates lacking these genes. ► Genetic deletion of Pfhrp2 and Pfhrp3 may lead to false negative results in RDTs. Genetic polymorphisms in diagnostic antigens are important factors responsible for variable performance of rapid diagnostic tests. Additionally, the failure of antigen expression due to gene deletion may also contribute to variable performance. We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes leading to false negative results of rapid diagnostic tests. The study highlights need to determine the prevalence of P. falciparum isolates lacking these genes in larger field populations in India.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2012.09.015