RNA Aptamers Inhibit the Growth of the Fish Pathogen Viral Hemorrhagic Septicemia Virus (VHSV)

Viral hemorrhagic septicemia virus (VHSV) is a serious disease impacting wild and cultured fish worldwide. Hence, an effective therapeutic method against VHSV infection needs to be developed. Aptamer technology is a new and promising method for diagnostics and therapeutics. It revolves around the us...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2012-12, Vol.14 (6), p.752-761
Main Authors: Punnarak, Porntep, Santos, Mudjekeewis D, Hwang, Seong Don, Kondo, Hidehiro, Hirono, Ikuo, Kikuchi, Yo, Aoki, Takashi
Format: Article
Language:English
Subjects:
Animals
Antiviral Agents - administration & dosage
Aptamers, Nucleotide - administration & dosage
Aptamers, Nucleotide - chemistry
Aquaculture
Biomedical and Life Sciences
Biotechnology
cytopathogenicity
electrophoresis
Engineering
evolution
Flounder
Flounder - virology
Freshwater & Marine Ecology
Genotype & phenotype
growth retardation
Hemorrhagic Septicemia, Viral - drug therapy
Hemorrhagic Septicemia, Viral - virology
Infections
Laboratories
Life Sciences
Ligands
Marine
Methods
Microbiology
Monoclonal antibodies
new technology
Novirhabdovirus - chemistry
Novirhabdovirus - drug effects
Novirhabdovirus - physiology
oligonucleotides
Original Article
Paralichthys olivaceus
Pathogens
Peptides
Pleuronectiformes
Proteins
Rhodovulum sulfidophilum
Ribonucleic acid
RNA
RNA polymerase
Sepsis
therapeutics
Treatment Outcome
Viral hemorrhagic septicemia virus
Viruses
Zoology
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Summary:Viral hemorrhagic septicemia virus (VHSV) is a serious disease impacting wild and cultured fish worldwide. Hence, an effective therapeutic method against VHSV infection needs to be developed. Aptamer technology is a new and promising method for diagnostics and therapeutics. It revolves around the use of an aptamer molecule, an artificial ligand (nucleic acid or protein), which has the capacity to recognize target molecules with high affinity and specificity. Here, we aimed at selecting RNA aptamers that can specifically bind to and inhibit the growth of a strain of fish VHSV both in vitro and in vivo. Three VHSV-specific RNA aptamers (F1, F2, and C6) were selected from a pool of artificially and randomly produced oligonucleotides using systematic evolution of ligands by exponential enrichment. The three RNA aptamers showed obvious binding to VHSV in an electrophoretic mobility shift assay but not to other tested viruses. The RNA aptamers were tested for their ability to inhibit VHSV in vitro using hirame natural embryo (HINAE) cells. Cytopathic effect and plaque assays showed that all aptamers inhibited the growth of VHSV in HINAE cells. In vivo tests using RNA aptamers produced by Rhodovulum sulfidophilum showed that extracellular RNA aptamers inhibited VHSV infection in Japanese flounder. These results suggest that the RNA aptamers are a useful tool for protection against VHSV infection in Japanese flounder.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-012-9448-1