Tissue and temporal expression of miR172 paralogs and the AP2-like target in oil palm (Elaeis guineensis Jacq.)

Oil palm is an economically important crop, and floral development is a critical process in oil palm productivity. MicroRNA172 (miR172) has been shown to be an important regulator of the APETALA2 (AP2)-like family transcription factors involved in floral timing in a number of plants and belongs to a...

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Bibliographic Details
Published in:Tree genetics & genomes 2012-12, Vol.8 (6), p.1331-1343
Main Authors: Mehrpooyan, F, Othman, R. Y, Harikrishna, J. A
Format: Article
Language:English
Subjects:
Bioinformatics
Biomedical and Life Sciences
Biotechnology
complementary DNA
Conserved sequence
Crops
Data processing
Development
Elaeis guineensis
expressed sequence tags
flowering
Forestry
Genes
Genetics
Genomes
Homology
Life Sciences
microRNA
MicroRNAs
miRNA
Oil
Original Paper
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Polymerase chain reaction
Productivity
sequence homology
Transcription factors
Tree Biology
Trees
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Summary:Oil palm is an economically important crop, and floral development is a critical process in oil palm productivity. MicroRNA172 (miR172) has been shown to be an important regulator of the APETALA2 (AP2)-like family transcription factors involved in floral timing in a number of plants and belongs to a group of highly conserved microRNA that can be identified based on sequence homology. We identified six oil palm miR172 paralogs, representing two distinct mature miR172 isoforms based on sequence identity and predicted hairpin structures and an AP2-like target, using a combination of RT-PCR cloning and bioinformatic analysis of oil palm expressed sequence tags data. Although oil palm miR172 cleavage products were detected through 5′-rapid amplification of cDNA ends, mature miR172a and the AP2-like target showed overlapping expression patterns in oil palm. Some variation in the expression of the six paralog miR172 precursors in different oil palm tissues was observed with each of the two mature isoforms showing different expression patterns during floral development. It is suggested that the expression of different miR172 precursor paralogs is tissue-specific, and for at least one mature miR172 isoform in oil palm, there may be significant cleavage of the AP2-like target.
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-012-0519-2