Modulation of pro-inflammatory mediators in LPS-stimulated human periodontal ligament cells by chitosan and quaternized chitosan

► Chitosan stimulated the proliferation of HPDLCs at 1d, 3d and 5d. ► Quaternized chitosan improved the proliferation at 1μg/mL concentrations after 5d culture. ► Chitosan inhibited the production of IL-1β and TNF-α at 24, 48 and 72h. ► Quaternized chitosan significantly increased IL-1β and TNF-α pr...

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Bibliographic Details
Published in:Carbohydrate polymers 2013-01, Vol.92 (1), p.824-829
Main Authors: Ji, Qiuxia, Deng, Jing, Yu, Xinbo, Xu, Quanchen, Wu, Hong, Pan, Jianfeng
Format: Article
Language:English
Subjects:
Applied sciences
bioactive properties
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Cell Proliferation - drug effects
Cells, Cultured
Chitosan
Chitosan - analogs & derivatives
Chitosan - chemistry
Chitosan - pharmacology
enzyme-linked immunosorbent assay
Exact sciences and technology
Fibroblast Growth Factors - antagonists & inhibitors
Fibroblast Growth Factors - metabolism
Humans
Inflammation Mediators - metabolism
Interleukin (IL)-1β
interleukin-1beta
Interleukin-1beta - antagonists & inhibitors
Interleukin-1beta - metabolism
ligaments
Lipopolysaccharides - pharmacology
LPS
Medical sciences
Natural polymers
Periodontal disease
Periodontal Diseases - drug therapy
Periodontal Diseases - metabolism
Periodontal Ligament - cytology
Periodontal Ligament - drug effects
Pharmacology. Drug treatments
Physicochemistry of polymers
Quaternized chitosan (HTCC)
Starch and polysaccharides
Tumor necrosis factor alpha (TNF-α)
tumor necrosis factor-alpha
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - metabolism
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Summary:► Chitosan stimulated the proliferation of HPDLCs at 1d, 3d and 5d. ► Quaternized chitosan improved the proliferation at 1μg/mL concentrations after 5d culture. ► Chitosan inhibited the production of IL-1β and TNF-α at 24, 48 and 72h. ► Quaternized chitosan significantly increased IL-1β and TNF-α production at 24, 48 and 72h. ► The production of IL-1β and TNF-α was inhibited by bFGF. The aim of this study was to evaluate the effects of chitosan and quaternized chitosan (HTCC) modulate IL-1β and TNF-α in LPS-stimulated human periodontal ligament cells (HPDLCs). MTT assay revealed that chitosan stimulated the proliferation of HPDLCs. However, HTCC inhibited the proliferation of HPDLCs at concentrations of 1000 and 100μg/mL more than the control, especially after 5d (P
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2012.09.043