Effect of extenders on sperm mitochondrial membrane, plasma membrane and sperm kinetics during liquid storage of canine semen at 5°C

The objective of this study was to evaluate the effect of addition of egg yolk (EY) or soy lecithin (SL) based extenders on dog sperm parameters during 10days storage at 5°C. Four ejaculates of pooled semen from three Mongrel dogs were divided into three aliquots and extended to a final concentratio...

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Bibliographic Details
Published in:Animal reproduction science 2012-12, Vol.136 (1-2), p.139-145
Main Authors: Kasimanickam, Vanmathy R, Kasimanickam, Ramanathan K, Memon, Mushtaq A, Rogers, Harmon A
Format: Article
Language:English
Subjects:
Animals
Cell Membrane - drug effects
Chilled semen
Computer assisted sperm analyzer
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Dog
Dogs
egg yolk
Male
membrane potential
Mitochondria
Mitochondrial Membranes - drug effects
Plasma membrane
semen
Semen Preservation - veterinary
Specimen Handling
Sperm kinetics
sperm motility
Sperm Motility - drug effects
Sperm Motility - physiology
spermatozoa
Spermatozoa - cytology
Spermatozoa - drug effects
Spermatozoa - physiology
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Summary:The objective of this study was to evaluate the effect of addition of egg yolk (EY) or soy lecithin (SL) based extenders on dog sperm parameters during 10days storage at 5°C. Four ejaculates of pooled semen from three Mongrel dogs were divided into three aliquots and extended to a final concentration of 200 million sperm/mL using following non-commercial extenders: egg yolk extender (EYE) group – semen+20% Tris-EYE; soy lecithin (SL) group 1 – semen+SL extender with 0.04% SL; SL group 2 – semen+SL extender 0.4% SL. The extended semen samples were stored at 5°C and were evaluated for sperm mitochondrial membrane potential (MMP), plasma membrane integrity (%PMI) and computer assisted sperm analyzer (CASA) sperm motility parameters on days 0, 1, 2, 3, 4, 7 and 10. The extender, days of storage and extender×days of storage affected the MMP, %PMI and CASA sperm motility parameters (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2012.10.024