Embryo incubation and selection in a time-lapse monitoring system improves pregnancy outcome compared with a standard incubator: a retrospective cohort study

Objective To quantify the effect on reproductive outcome of culturing and selecting embryos using a novel time-lapse monitoring system (TMS). Design Retrospective observational cohort study. Setting University-affiliated private center. Patient(s) Donation and autologous intracytoplasmic sperm injec...

Full description

Saved in:
Bibliographic Details
Published in:Fertility and sterility 2012-12, Vol.98 (6), p.1481-1489.e10
Main Authors: Meseguer, Marcos, Ph.D, Rubio, Irene, Ph.D, Cruz, Maria, Ph.D, Basile, Natalia, Ph.D, Marcos, Julian, Ph.D, Requena, Antonio, M.D
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
clinical pregnancy
cohort studies
confidence interval
culture system
Embryo development
embryo selection
embryo transfer
Embryo Transfer - methods
Embryo Transfer - statistics & numerical data
Female
Fertility Preservation - methods
Fertility Preservation - statistics & numerical data
Fertilization in Vitro - methods
Fertilization in Vitro - statistics & numerical data
Gynecology. Andrology. Obstetrics
Humans
Incubators
Internal Medicine
intracytoplasmic sperm injection
Medical sciences
Middle Aged
monitoring
morphokinetic parameters
Obstetrics and Gynecology
odds ratio
oocytes
patients
Pregnancy
pregnancy outcome
Pregnancy Rate
Prevalence
regression analysis
Spain - epidemiology
Time-Lapse Imaging - methods
Time-Lapse Imaging - statistics & numerical data
time-lapse monitoring
Treatment Outcome
ultrasonics
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective To quantify the effect on reproductive outcome of culturing and selecting embryos using a novel time-lapse monitoring system (TMS). Design Retrospective observational cohort study. Setting University-affiliated private center. Patient(s) Donation and autologous intracytoplasmic sperm injection (ICSI) cycles from ten IVF clinics using similar procedures, cultured in TMS (n = 1,390) or in a standard incubator (SI; n = 5,915). Intervention(s) None. Main Outcome Measure(s) Clinical pregnancy rate confirmed by ultrasound in week 7. Result(s) A logistic regression analysis, which included all significant confounding factors, was used to evaluate the effect of culturing and selecting embryos with the use of TMS. Comparing clinical pregnancy rates per oocyte retrieval with TMS and SI treatments gave a crude effect of odds ratio [OR] 1.190 (95% confidence interval [CI] 1.058–1.337). Oocyte source, maternal age, day of transfer, and number of retrieved oocytes were identified as significant confounding factors. After accounting for confounding factors, the effect of TMS culture was OR 1.201 (95% CI 1.059–1.363). Limiting analysis to treatments with embryo transfer and including number of transferred embryos as a confounding factor likewise gave a significant effect of TMS with OR 1.157 (95% CI 1.018–1.315). Conclusion(s) Analysis of retrospective data indicated that culturing and selecting embryos by TMS significantly improved the relative probability of clinical pregnancy (+20.1% per oocyte retrieval, +15.7% per embryo transfer). The elevated clinical pregnancy rate was attributed to a combination of stable culture conditions and the use of morphokinetic parameters for embryo selection.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2012.08.016