On the role of extracellular polymeric substances during early stages of Xylella fastidiosa biofilm formation

[Display omitted] ► SEM and AFM images suggest EPS production during all stages of biofilm formation. ► IR–ATR spectra reveal EPS production since early stages of X. fastidiosa biofilm. ► Our results suggest EPS is extensively involved in irreversible adhesion processes. The structural integrity and...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-02, Vol.102, p.519-525
Main Authors: Lorite, Gabriela S., de Souza, Alessandra A., Neubauer, Daniel, Mizaikoff, Boris, Kranz, Christine, Cotta, Mônica A.
Format: Article
Language:English
Subjects:
AFM
Biofilm
Biofilms - growth & development
Extracellular polymeric substances
IR–ATR
Polymers - chemistry
Polymers - metabolism
Spectroscopy, Fourier Transform Infrared
Xylella - growth & development
Xylella - metabolism
Xylella fastidiosa
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Summary:[Display omitted] ► SEM and AFM images suggest EPS production during all stages of biofilm formation. ► IR–ATR spectra reveal EPS production since early stages of X. fastidiosa biofilm. ► Our results suggest EPS is extensively involved in irreversible adhesion processes. The structural integrity and protection of bacterial biofilms are intrinsically associated with a matrix of extracellular polymeric substances (EPS) produced by the bacteria cells. However, the role of these substances during biofilm adhesion to a surface remains largely unclear. In this study, the influence of EPS on Xylella fastidiosa biofilm formation was investigated. This bacterium is associated with economically important plant diseases; it presents a slow growth rate and thus allows us to pinpoint more precisely the early stages of cell-surface adhesion. Scanning electron microscopy and atomic force microscopy show evidence of EPS production in such early stages and around individual bacteria cells attached to the substrate surface even a few hours after inoculation. In addition, EPS formation was investigated via attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR). To this end, X. fastidiosa cells were inoculated within an ATR liquid cell assembly. IR–ATR spectra clearly reveal EPS formation already during the early stages of X. fastidiosa biofilm formation, thereby providing supporting evidence for the hypothesis of the relevance of the EPS contribution to the adhesion process.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2012.08.027