A facile and green ultrasonic-assisted synthesis of BSA conjugated silver nanoparticles

[Display omitted] ► Reduction of Ag ions to produce Ag nanoparticles with simultaneous capping. ► Ultrasonic assistance leads to Ostwald ripening. ► The size and shape of nanoparticles can be controlled. ► Greener process as it uses aqueous media. ► Creates hybrid material consisting of an inorganic...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-02, Vol.102, p.879-883
Main Authors: Gautam, Saurabh, Dubey, Priyanka, Gupta, Munishwar N.
Format: Article
Language:English
Subjects:
Animals
BSA capped nanoparticles
Cattle
circular dichroism spectroscopy
colloids
Green Chemistry Technology
Green synthesis
ions
light scattering
Metal Nanoparticles - chemistry
nanoparticles
nanosilver
Ostwald ripening
Serum Albumin, Bovine - chemistry
Shape controlled synthesis
silver
Silver - chemistry
Silver nanoparticles
Surface Plasmon Resonance
thiols
transmission electron microscopy
ultrasonic treatment
Ultrasonics
zeta potential
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Summary:[Display omitted] ► Reduction of Ag ions to produce Ag nanoparticles with simultaneous capping. ► Ultrasonic assistance leads to Ostwald ripening. ► The size and shape of nanoparticles can be controlled. ► Greener process as it uses aqueous media. ► Creates hybrid material consisting of an inorganic and a biological material. The formation and growth of hybrid nanoparticles of a protein BSA and silver by ultrasonic assistance were tracked by surface plasmon resonance signal of silver nanoparticles and light scattering. The hybrid nanoparticles were characterized by surface plasmon resonance spectra, light scattering, TEM, circular dichroism spectroscopy and zeta potential. Size along with the spherical shape of the nanoparticles could be controlled and nanoparticles with diameters ranging from 8 to 140nm could be obtained, depending upon the ultrasonication time (15–30min) and molar ratio of AgNO3/BSA (20–200). The role of single free thiol group in the reduction of silver ions was also investigated by using DTNB modified BSA and protein conjugated silver nanoparticles were formed even with thiol modified BSA. The growth and size of the nanoparticles were governed by ultrasonic assisted Ostwald ripening. BSA conjugated with silver nanoparticles showed changes in the secondary structure with an increase in the beta sheet structure to 33% as compared to 7% in native BSA as determined by CD spectra. Zeta potential measurements in the pH range of 2.0–12.0 demonstrated that the surface charges of the BSA conjugated silver nanoparticles were similar to that of native BSA suggesting that surface charges and overall three dimensional structure of BSA did not change much. This approach provides a strategy for completely green synthesis of hybrid nanoparticles consisting of a biological entity and an inorganic material. This is the first application of ultrasonic assistance in formation of such hybrid nanomaterials in aqueous media.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2012.10.007